A Sensitive Resonance Scattering Spectral Assay for the Determination of Trace H2O2 Based on the HRP Catalytic Reaction and Nanogold Aggregation
A Sensitive Resonance Scattering Spectral Assay for the Determination of Trace H2O2 Based on the HRP Catalytic Reaction and Nanogold Aggregation
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DOI：
10.1007/s10895-008-0328-z
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发表时间：
2008-02
影响因子：
2.7
通讯作者：
Aihui Liang;N. Zhang;Zhiliang Jiang;Su-mei Wang
中科院分区：
文献类型：
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作者：
Aihui Liang;N. Zhang;Zhiliang Jiang;Su-mei Wang
在pH 6.4的柠檬酸盐缓冲溶液中，粒径为15 nm的金纳米粒子在580 nm处产生共振散射（RS）峰。辣根过氧化物酶（HRP）强烈催化过氧化氢氧化邻苯二胺生成环己-3，5-二烯-1，2-二亚基二胺和2，3-二氨基吩嗪的中间产物。这两种方法都能使金纳米粒子聚集，使580 nm处的拉曼光谱强度大大增强。在最佳条件下，H_2O_2（C）浓度在0.08-2.2 μmol/l范围内与580 nm处增强的RS强度（ΔI580 nm）成正比。其回归方程为ΔI580 nm= 46.8C+ 3.4，相关系数为0.9983，检出限为0.03 μmol/l H2 O2。该方法用于水样中H2 O2的测定，结果满意。
Gold nanoparticles in size of 15 nm exhibit a resonance scattering (RS) peak at 580 nm, in pH 6.4 citrate buffer solutions. Horseradish peroxidase (HRP) strongly catalyzed the H2O2oxidation ofo-phenylenediamine to form an intergradation of cyclohexa-3, 5-diene-1, 2-diylidenediamine and the product of 2, 3-diaminophenazin. Both cause gold nanoparticles aggregations to enhance the RS intensity at 580 nm greatly. Under the optimal conditions, the concentration of H2O2(C) in the range of 0.08–2.2 μmol/l was proportional to the enhanced RS intensity at 580 nm (ΔI580 nm). Its regression equation was ΔI580 nm= 46.8C+ 3.4, with a correlation coefficient of 0.9983 and a detection limit of 0.03 μmol/l H2O2. This new RS spectral method was applied to the determination of H2O2in water samples with satisfactory results.