Plaque-associated endothelial dysfunction in apolipoprotein E-deficient mice on a regular diet. Effect of human apolipoprotein AI
Plaque-associated endothelial dysfunction in apolipoprotein E-deficient mice on a regular diet. Effect of human apolipoprotein AI
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DOI：
10.1016/s0008-6363(03)00353-5
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发表时间：
2003-07-01
影响因子：
10.8
通讯作者：
Bult, H
中科院分区：
文献类型：
--
作者：
Crauwels, HM;Van Hove, CE;Bult, H
目的：正常饮食的载脂蛋白E缺陷小鼠（apoE（-/-））会出现高胆固醇血症并发展为动脉粥样硬化，但内皮依赖性舒张功能在长达6个月的时间内保持不受干扰。我们研究了老年人apoE（-/-）是否会发生血管功能障碍。缺陷是系统性的（高胆固醇血症依赖性的）还是局灶性的（斑块相关的），以及人载脂蛋白AI转基因（apoAI/E-/-）的影响。研究方法：将apoE（-/-）（n=5）、apoAI/E-/-（n=6）和C57 B16/6 J（WT，n=4）小鼠（18个月）的动脉系统地解剖用于等长张力记录和随后的形态测量。结果：乙酰胆碱（ACh）诱导的舒张功能受损（P
Objective: Apolipoprotein E-deficient mice (apoE(-/-)) on a regular diet become hypercholesterolemic and develop atherosclerosis, but endothelium-dependent relaxation remains undisturbed for up to 6 months. We investigated whether vasomotor dysfunction develops in aged apoE(-/-). whether the defect was systemic (hypercholesterolemia-dependent) or focal (plaque-related), and the effect of human apolipoprotein AI transgenesis (apoAI/E-/-). Methods: Arteries of apoE(-/-) (n=5), apoAI/E-/- (n=6) and C57B16/6J (WT, n=4) mice (18 months) were systematically dissected for isometric tension recording and subsequent morphometry. Results: Acetylcholine (ACh)-induced relaxation was impaired (P