FUNCTIONAL EXPRESSION OF THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM IN THE YEAST SACCHAROMYCES-CEREVISIAE
FUNCTIONAL EXPRESSION OF THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM IN THE YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.7.6.2087
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发表时间：
1987-06-01
影响因子：
5.3
通讯作者：
SAUER, B
中科院分区：
文献类型：
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作者：
SAUER, B
原核噬菌体P1的crer -lox位点特异性重组系统在真核生物酵母（酿酒酵母）中有效地发挥作用。编码位点特异性重组酶的cre基因被置于酵母GAL1启动子的控制之下。LEU2基因两侧的lox位点在两个方向上被整合到两个不同的染色体上。在lox位点的重组（通过LEU2基因的缺失来测量）被Cre蛋白有效而准确地促进，并且依赖于半乳糖的诱导。这些结果表明，原核重组酶可以进入真核生物细胞核，而且，Cre重组酶在酿酒酵母染色体上进行精确重组的能力不受染色质结构的影响。
The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GAL1 promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.