Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant
Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant
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苦荞 APETALA1 直向同源物的异位表达仅拯救拟南芥 ap1 突变体的萼片发育
DOI：
10.3390/ijms20082021
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发表时间：
2019-04-02
影响因子：
5.6
通讯作者：
Fang, Zhengwu
中科院分区：
文献类型：
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作者：
Liu, Zhixiong;Fei, Yue;Fang, Zhengwu
荞麦（Fagopyrum esculentum）（蓼科：野荞麦目）具有未分化的花被，包括5个艳丽的花被片，这与大多数核心真双子叶植物中分化为典型萼片和花瓣的花被片不完全对应。在拟南芥中，APETALA 1（AP 1）基因参与指定萼片和花瓣发育。本研究从F.蔬菜FaesAP 1的表达主要是在所有花器官中检测到的，并且在针式花和线式花中当花被片快速伸长时维持在高水平。此外，pFaesAP 1驱动的GUS报告基因在萼片密集但花瓣很弱或不存在的花中被激活。此外，拟南芥ap 1 -10突变体中的FaesAP 1异位表达完全拯救了萼片发育，明显促进了早期开花，但未能补充花瓣发育。本研究提供的证据表明，F。esculentum与核心真双子叶植物的萼片同源。此外，这些研究结果表明，不同的花被身份程序在Carlisllales，这表明，AP 1直系同源物参与花瓣发育可能独立地跨越不同的分支的核心真双子叶植物。我们的研究结果还表明，FaesAP 1具有潜在的生物技术工程，以开发早期开花品种的F。蔬菜
Fagopyrum esculentum (Polygonaceae: Caryophyllales) exhibits an undifferentiated perianth comprising five showy tepals, which does not completely correspond to the perianth differentiated into typical sepals and petals in most core eudicots. In Arabidopsis, the APETALA1 (AP1) gene is involved in specifying sepals and petals development. Here we isolated AP1 ortholog, FaesAP1, and a 2.2kb FaesAP1 promoter (pFaesAP1) from F. esculentum. FaesAP1 expression is mainly detectable in all floral organs and maintains at a high level when tepals elongate rapidly both in pin and thrum flowers. Moreover, the GUS reporter gene driven by pFaesAP1 was activated in flowers where the sepals were intense, but the petals very weak or absent. Additionally, FaesAP1 ectopic expression in Arabidopsis ap1-10 mutant rescues sepal development fully, obviously prompting early flowering, but failing to complement petal development. In this study, evidence was provided that the showy tepals in the F. esculentum are homologs to core eudicots sepals. Furthermore, these findings show a different perianth identity program in Caryophyllales, suggesting that AP1 orthologs involved in petal development may evolve independently across different clades of core eudicots. Our results also suggest that FaesAP1 holds potential for biotechnical engineering to develop early flowering varieties of F. esculentum.