The involvement of P2X3 receptors of rat sympathetic ganglia in cardiac nociceptive transmission
The involvement of P2X3 receptors of rat sympathetic ganglia in cardiac nociceptive transmission
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DOI：
10.1007/bf03165788
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发表时间：
2007-09
影响因子：
3.4
通讯作者：
C. P. Zhang;C. Xu;S. Liang;G. L. Li;Y. Gao;Y. X. Wang;A. Zhang;F. Wan
中科院分区：
文献类型：
--
作者：
C. P. Zhang;C. Xu;S. Liang;G. L. Li;Y. Gao;Y. X. Wang;A. Zhang;F. Wan
本研究通过P2X3受体拮抗剂A-317491对未成熟大鼠和心肌缺血大鼠颈上神经节（SCG） P2X3表达的影响，观察P2X3受体在心脏伤害性传递中的作用。改善了伤害行为。在心肌缺血损伤后14天的大鼠神经节神经元中，心肌缺血组P2X3受体的染色比未缺血组明显增强。心肌缺血大鼠经A-317491处理后，P2X3免疫反应强度低于心肌缺血大鼠。western blotting和原位杂交检测心肌缺血组P2X3及其蛋白和mRNA的信号均高于对照组。心肌缺血大鼠经A-317491处理后，P2X3及其mRNA的表达强度低于心肌缺血大鼠。上述结果提示P2X3受体参与心脏伤害感受传递，A-317491可能抑制心肌缺血后大鼠SCG中P2X3受体介导的传递。
In this work we have examined the effects of P2X3 receptor antagonist A-317491 on P2X3 expression in superior cervical ganglion (SCG) from naive and myocardial ischemic rats to observe the effect of P2X3 receptors in cardiac nociceptive transmission. A-317491 improved nociceptive behavior. In the ganglia neurons of rats at 14 days after myocardial ischemic injury, the staining of P2X3 receptor in myocardial ischemic groups appeared to be more intense than those of naive rats detected by immunohistochemistry. After myocardial ischemic rats treated with A-317491, the intensity of the P2X3 immunoreactivity was lower than that in myocardial ischemic rats. The signals of P2X3 and its protein and mRNA in myocardial ischemic groups were higher than those in control group measured by western blotting and in situ hybridization. After myocardial ischemic rats treated with A-317491, the intensity of the P2X3 and its mRNA was lower than that in myocardial ischemic rats. These results suggest the involvement of P2X3 receptors in cardiac nociceptive transmission and A-317491 may inhibit the transmission mediated by P2X3 receptors in rat SCG after myocardial ischemia.