Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases
Quantum Yields and Kinetics of the Firefly Bioluminescence Reaction of Beetle Luciferases
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DOI：
10.1111/j.1751-1097.2010.00777.x
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发表时间：
2010-09-01
影响因子：
3.3
通讯作者：
Ohmiya, Yoshihiro
中科院分区：
文献类型：
--
作者：
Niwa, Kazuki;Ichino, Yoshiro;Ohmiya, Yoshihiro
确定了来自发出不同生物发光颜色的发光甲虫的三个主要家族的甲虫荧光素酶的萤火虫生物发光反应的量子产率。量子产率（QY）与发光光谱显着相关。巴西点击甲虫 Pyrearinus termitluminans 的绿光发射荧光素酶，其发光光谱在所有研究的荧光素酶中具有最短的峰值波长，具有最高的 QY（0.61）。对活性位点取代的 Pyrocoelia miyako 荧光素酶的突变分析表明，虽然 k(cat) 因突变而降低，但 QY 并未受到显着影响。
Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected.