Electrochemical Detection of Platinum(IV) Prodrug Satraplatin in Serum.
Electrochemical Detection of Platinum(IV) Prodrug Satraplatin in Serum.
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血清中铂 (IV) 前药 Satraplatin 的电化学检测。
DOI：
10.1021/acs.analchem.5b03215
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发表时间：
2015
影响因子：
7.4
通讯作者：
Lai,RebeccaY
中科院分区：
文献类型：
--
作者：
Wu,Yao;Lai,RebeccaY
我们报道了一种无试剂和可重复使用的电化学传感器的设计和制造，用于检测铂(IV)前体药物赛特铂(SAT)。该检测策略是基于SAT的铂(IV)中心与表面固定的亚甲基蓝之间的电催化反应。我们系统地评估了钝化稀释剂链长对传感器整体性能的影响。我们的结果表明，使用较短的稀释剂，如2-巯基乙醇，比使用较长和更钝化的稀释剂，如6-巯基-1-己醇更有利。除了使用循环伏安法或计时电流法作为传感器询问技术外，所有三个传感器，每个都用不同的烷硫醇稀释剂钝化，已被证明是敏感的；检测限在1-10μM的范围内。它们也具有高度的特异性，对顺铂和卡铂等铂(II)药物不起作用。更重要的是，它们具有足够的选择性，可以直接用于50%的血清。该传感策略在临床药代动力学研究中具有潜在的应用前景。
We report the design and fabrication of a reagentless and reusable electrochemical sensor for detection of satraplatin (SAT), a platinum(IV) prodrug. The detection strategy is based on the electrocatalytic reaction between the Pt(IV) center of SAT and surface-immobilized methylene blue. We systematically evaluated the effect of passivating diluent chain length on the overall sensor performance. Our results show that the use of a shorter diluent like 2-mercaptoethanol is more advantageous than using a longer and more passivating diluent such as 6-mercapto-1-hexanol. Independent of the use of cyclic voltammetry or chronoamperometry as the sensor interrogation technique, all three sensors, each passivated with a different alkanethiol diluent, have been demonstrated to be sensitive; the limit of detection is in the range of 1–10 μM. They are also highly specific and do not respond to Pt(II) drugs such as cisplatin and carboplatin. More importantly, they are selective enough to be employed directly in 50% serum. This sensing strategy has potential applications in clinical pharmacokinetics studies.