Characterization of extracellular polymeric substances and microbial diversity in anaerobic co-digestion reactor treated sewage sludge with fat, oil, grease
Characterization of extracellular polymeric substances and microbial diversity in anaerobic co-digestion reactor treated sewage sludge with fat, oil, grease
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厌氧共消化反应器处理脂肪、油、油脂污水污泥中胞外聚合物和微生物多样性的表征
DOI：
10.1016/j.biortech.2016.04.046
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发表时间：
2016
影响因子：
11.4
通讯作者：
Li Min
中科院分区：
文献类型：
--
作者：
Yang Zhao-Hui;Xu Rui;Zheng Yue;Chen Ting;Zhao Li-Jun;Li Min
用脂肪、油和油脂 (FOG) 处理污水污泥 (SS) 的共消化器的性能在两个中温反应器中半连续进行了 180 天。与SS单一消化相比，通过添加上限FOG（VS上60％），共消化的沼气产量和TS去除效率显着提高，分别达到35％和26％。细胞外聚合物 (EPS) 的释放也刺激了共消化性能的增强，与单一消化器相比，松散结合 EPS (LB-EPS) 和紧密结合 EPS (TB-EPS) 中的释放增加了 40%。基于高通量测序（HTS），对微生物16S rRNA基因进行分析，全面揭示微生物群落的动态变化。结果表明，加入FOG后，细菌和古菌都经历了明显的演替，并且大量的菌群如Methanosaeta和N09参与了这一过程。冗余分析表明，乙酸碎屑属Methanosaeta与沼气产生和EPS降解明显相关。
Performance of co-digesters, treated of sewage sludge (SS) with fat, oil and grease (FOG), were conducted semi-continuously in two mesophilic reactors over 180 days. Compared with SS mono-digestion, biogas production and TS removal efficiency of co-digestion were significantly enhanced up to 35% and 26% by adding upper limit FOG (60% on VS). Enhancement in co-digestion performance was also stimulated by the release of extracellular polymeric substances (EPS), which was increased 40% in both loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) than that of mono-digester. Based on high-throughput sequencing (HTS), analysis of microbial 16S rRNA gene comprehensively revealed the dynamic change of microbial community. Results showed that both bacterial and archaeal undergone an apparent succession with FOG addition, and large amount of consortium likeMethanosaetaandN09were involved in the process. Redundancy analysis showed the acetoclastic generaMethanosaetadistinctly related with biogas production and EPS degradation.