CLONING OF HUMAN MICROTUBULE-ASSOCIATED PROTEIN 1B AND THE IDENTIFICATION OF A RELATED GENE ON CHROMOSOME-15
CLONING OF HUMAN MICROTUBULE-ASSOCIATED PROTEIN 1B AND THE IDENTIFICATION OF A RELATED GENE ON CHROMOSOME-15
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DOI：
10.1006/geno.1994.1384
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发表时间：
1994-07-15
期刊：
影响因子：
4.4
通讯作者：
KUNKEL, LM
中科院分区：
文献类型：
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作者：
LIEN, LL;FEENER, CA;KUNKEL, LM
我们报道了人微管相关蛋白1B(MAP1B)的完整克隆和序列分析。与小鼠和大鼠MAP1B部分序列的比较表明，该基因非常保守，分别有91%和90%的同源性。人类MAP1B全基因组区已被分离并确定了基因组组织，该基因包括7个外显子，第三个外显子包含小鼠和大鼠MAP1B中没有的序列。这个标记为3A的序列存在于另一种转录本的5‘端，该转录本的表达水平约为全长转录本的十分之一。通过人MAP1B与序列数据库的比较，我们鉴定了一个与MAP1B相关的基因，它可能是大鼠MAP1A的人类同源物。该基因在大脑和脊髓中高水平表达，在肌肉中低水平表达，并定位于人类15号染色体的长臂。(C)1994年学术出版社，Inc.
We report here the complete cloning and sequencing of human microtubule associated protein 1B (MAP1B). Comparisons to mouse and partial rat MAP1B sequence indicate that this gene is extremely well conserved, with 91 and 90% identity, respectively. The entire human MAP1B genomic region has been isolated and the genomic organization determined, The gene includes seven exons, and the third exon contains sequence not represented in mouse or rat MAP1B. This sequence, labeled 3A, is present at the 5' end of an alternative transcript that is expressed at approximately 1/10th the level of the full-length transcript. By comparisons of human MAP1B with the sequence databases, we have identified a MAP1B-related gene that is probably the human homologue of rat MAP1A. This gene is expressed at high levels in brain and spinal cord and much lower levels in muscle and maps to the long arm of human chromosome 15. (C) 1994 Academic Press, Inc.