Proteins and glycoproteins in plasma membranes and in the membrane lamellae produced by purified oligodendroglia in culture.
Proteins and glycoproteins in plasma membranes and in the membrane lamellae produced by purified oligodendroglia in culture.
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培养物中纯化的少突胶质细胞产生的质膜和膜层中的蛋白质和糖蛋白。
DOI：
10.1016/0005-2736(83)90436-4
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Poduslo,SE
中科院分区：
文献类型：
--
作者：
Poduslo,SE
少突胶质细胞质膜是由蛋白质和糖蛋白的异质混合物组成的复杂结构。考马斯染色凝胶图案显示最多 40 种蛋白质，分子量范围从 > 200 000 到 12 500。放射自显影用于检测放射性碘化凝集素与糖蛋白的结合。对于伴刀豆球蛋白 A，观察到 5 种主要糖蛋白；小麦胚芽凝集素发现了两种主要糖蛋白，分子量约为95 000和78 000；在 Ulex europaeus 中，观察到 7 种主要糖蛋白。还看到了其他小带。使用不渗透性探针重氮二[125 I]碘磺胺酸来放射性标记完整细胞。结果发现，质膜中有 5 种主要蛋白质被放射性标记。在所有情况下，少突胶质细胞在培养物中产生的膜片螺旋往往具有稍微不那么复杂的模式，其蛋白质和糖蛋白比质膜更少。然而，膜片的螺纹具有比髓磷脂复杂得多的蛋白质模式。
Oligodendroglial plasma membranes are complex structures composed of a heterogeneous mixture of proteins and glycoproteins. The Coomassie stained gel patterns showed a maximum of 40 proteins with molecular weights ranging from> 200 000 to 12 500. Autoradiography was used to detect binding of radioiodinated lectins to glycoproteins. With concanavalin A, 5 major glycoproteins were seen; with wheat germ agglutinin, 2 major glycoproteins with approximate molecular weights of 95 000 and 78 000 were found; with Ulex europaeus, 7 major glycoproteins were observed. Additional minor bands were also seen. The impermeant probe diazodi [125 I] iodosulfanilic acid was used to radiolabel intact cells. It was found that 5 major proteins were radiolabeled in the plasma membranes. In all cases, the whorls of membrane lamellae produced in culture by oligodendroglia tend to have a somewhat less complicated pattern with fewer proteins and glycoproteins than the plasma membranes. However, the whorls of membrane lamellae have far more complicated protein patterns than myelin.