Functional Profiling of Human Fungal Pathogen Genomes
Functional Profiling of Human Fungal Pathogen Genomes
复制标题
DOI：
10.1101/cshperspect.a019596
复制
发表时间：
2015-03-01
影响因子：
5.4
通讯作者：
Madhani, Hiten D.
中科院分区：
文献类型：
--
作者：
Goranov, Alexi I.;Madhani, Hiten D.
真菌感染的诊断具有挑战性，通常难以治疗，只有少数药物类别存在。了解致病真菌引起人类疾病的分子机制是当务之急。在这里，我们讨论了如何开发和使用基因组规模的遗传资源，如全基因组敲除收集，可以解决这一未满足的需求。以酿酒隐球菌的研究为指导，对新型隐球菌和白色念珠菌的研究表明了如何克服大规模基因缺失的挑战，以及如何有效地利用这些集合来深入了解发病机制。我们的结论是，通过共同努力，人类真菌病原体基因组的全基因组功能分析是触手可及的。
Fungal infections are challenging to diagnose and often difficult to treat, with only a handful of drug classes existing. Understanding the molecular mechanisms by which pathogenic fungi cause human disease is imperative. Here, we discuss how the development and use of genome-scale genetic resources, such as whole-genome knockout collections, can address this unmet need. Using work in Saccharomcyes cerevisiae as a guide, studies of Cryptococcus neoformans and Candida albicans have shown how the challenges of large-scale gene deletion can be overcome, and how such collections can be effectively used to obtain insights into mechanisms of pathogenesis. We conclude that, with concerted efforts, full genome-wide functional analysis of human fungal pathogen genomes is within reach.