Environmental and genetic determinants of tobacco use: methodology for a multidisciplinary, longitudinal family-based investigation.
Environmental and genetic determinants of tobacco use: methodology for a multidisciplinary, longitudinal family-based investigation.
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发表时间：
2003-10
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通讯作者：
G. Swan;K. Hudmon;L. Jack;Kymberli K Hemberger;D. Carmelli;T. Khroyan;H. Ring;H. Hops;J. Andrews;E. Tildesley;D. McBride;N. Benowitz;C. Webster;K. Wilhelmsen;H. Feiler;B. Koenig;L. Caron;J. Illes;L. Cheng
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作者：
G. Swan;K. Hudmon;L. Jack;Kymberli K Hemberger;D. Carmelli;T. Khroyan;H. Ring;H. Hops;J. Andrews;E. Tildesley;D. McBride;N. Benowitz;C. Webster;K. Wilhelmsen;H. Feiler;B. Koenig;L. Caron;J. Illes;L. Cheng
本文描述了六个研究小组正在进行的合作努力，操作和执行一个综合的方法来研究烟草依赖的发展中的基因x环境相互作用。该项目的核心是对烟草使用的社会和行为风险因素进行纵向调查，这些人在摄入时平均年龄为13岁，并且之前已经描述了从青春期早期到成年早期的吸烟结果（队列的当前平均年龄为29岁）。提出了综合方法的概念框架和研究所依据的纵向调查。还描述了用于以下目的的方法：（a）招募参与者和家庭提供DNA样本和烟草使用信息;（B）评估参与者的相关烟草相关表型，包括吸烟史、当前烟草使用和尼古丁代谢;（c）评估从参与者收集的DNA样本的质量，以进行全基因组扫描和候选基因分析;（d）审查关于遗传和环境因素在开始和维持烟草使用中的作用的几个研究问题;（e）确保遵守地方和联邦准则，对家庭中基因型与烟草相关表型的关系进行伦理和法律的调查。这项调查是唯一的正在进行的研究中的烟草依赖的遗传学在何种程度上同等重要性已分配给表型和基因型的测量。
This article describes the ongoing collaborative effort of six research teams to operationalize and execute an integrative approach to the study of gene x environment interactions in the development of tobacco dependence. At the core of the project is a longitudinal investigation of social and behavioral risk factors for tobacco use in individuals who were, on average, 13 years of age at intake and for whom smoking outcomes extending from early adolescence to young adulthood have been characterized previously (current average age of the cohort is 29 years). The conceptual framework for the integrative approach and the longitudinal investigation on which the study is based is presented. A description is also provided of the methods used to: (a) recruit participants and families to provide DNA samples and information on tobacco use; (b) assess participants for relevant tobacco-related phenotypes including smoking history, current use of tobacco, and nicotine metabolism; (c) assess the quality of the DNA samples collected from participants for genome-wide scanning and candidate gene analysis; (d) examine several research questions concerning the role of genetic and environmental factors in the onset and maintenance of tobacco use; and (e) ensure adherence to local and federal guidelines for ethical and legal investigations of genotypic associations with tobacco-related phenotypes in families. This investigation is unique among ongoing studies of the genetics of tobacco dependence in the extent to which equal importance has been assigned to both phenotypic and genotypic measurements.