Expression of calbindin-D9k messenger ribonucleic acid in the gastrointestinal tract of dairy cattle.
Expression of calbindin-D9k messenger ribonucleic acid in the gastrointestinal tract of dairy cattle.
复制标题
钙结合蛋白-D9k 信使核糖核酸在奶牛胃肠道中的表达。
DOI：
10.1046/j.1439-0442.2002.00483.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Yamada
中科院分区：
文献类型：
--
作者：
N. Yamagishi;Y. Yukawa;N. Ishiguro;S. Soeta;I. Lee;K. Oboshi;H. Yamada
众所周知，怀孕和哺乳期的钙需求会上调肠道中 Calbindin-D9k (CaBP-9k) mRNA 的表达。奶牛胃肠道 CaBP-9k mRNA 表达尚未在奶牛中进行研究，因为奶牛必定会经历多个妊娠和哺乳阶段。在本研究中，通过 Northern blot 分析检测了荷斯坦奶牛胃肠道中 CaBP-9k mRNA 的表达。可检测到的 CaBP-9k mRNA 表达位于小肠近端部分。这些表达在十二指肠的最近端区域较高，并在远端逐渐降低。在0.4至83.4月龄的所有奶牛中均检测到十二指肠CaBP-9k mRNA，但在胎儿中未检测到。年龄和CaBP-9k mRNA表达水平之间以及血浆1,25-(OH)2D3浓度和CaBP-9k mRNA表达水平之间没有显着相关性。
The calcium demands of pregnancy and lactation are known to up-regulate expression of Calbindin-D9k (CaBP-9k) mRNA in the intestines. The gastrointestinal CaBP-9k mRNA expressions has not been studied in dairy cows, which are bound to experience several pregnancies and lactation stages. In this study, the CaBP-9k mRNA expression were examined in the gastrointestinal tract of Holstein dairy cattle by Northern blot analysis. Detectable expression of CaBP-9k mRNA was localized in the proximal portion of the small intestines. These expressions were higher at the most proximal region of the duodenum and gradually decreased distally. The duodenal CaBP-9k mRNA was detected in all dairy cattle from 0.4 to 83.4 months old, but was not detectable in foetuses. There were no significant correlations between the age and the levels of CaBP-9k mRNA expression or between the plasma 1,25-(OH)2D3 concentrations and the levels of CaBP-9k mRNA expression.
老年大鼠肠道钙吸收：肠道对 1,25(OH)2 维生素 D 抵抗的证据。
影响因子：
4.8
作者：
Wood,RJ;Fleet,JC;Cashman,K;Bruns,ME;Deluca,HF
通讯作者：
Deluca,HF