CD14+ cell-derived IL-29 modulates proinflammatory cytokine production in patients with allergic airway inflammation
CD14+ cell-derived IL-29 modulates proinflammatory cytokine production in patients with allergic airway inflammation
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DOI：
10.1111/j.1398-9995.2010.02455.x
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发表时间：
2011-02-01
期刊：
影响因子：
12.4
通讯作者：
Yang, P.
中科院分区：
文献类型：
--
作者：
He, S.;Li, T.;Yang, P.
背景：白细胞介素(IL)-29是一种新发现的具有抗病毒活性、诱导肿瘤细胞死亡和调节免疫功能的细胞因子。它是否在免疫紊乱中起作用尚不清楚。本研究旨在探讨IL-29在过敏环境下调节免疫应答中的作用。方法：本研究招募了一组患有过敏性哮喘或/和过敏性鼻炎的患者。在季节和非季节采集患者血清样本；采用酶联免疫分析法测定血清IL-29水平。免疫染色鉴定上气道黏膜产生il -29的细胞类型，免疫组织化学和流式细胞术检测。结果：变应性哮喘患者血清IL-29水平在季节性高，而在非季节性高。上呼吸道组织中IL-29+细胞以CD14+细胞居多。暴露于特定抗原触发抗原特异性CD4+ T细胞释放IL-4；释放的IL-4激活CD14+细胞释放IL-29；释放的IL-29进一步触发CD4+ T细胞IL-6和肿瘤坏死因子的释放。结论：白细胞介素-29通过调节免疫细胞释放促炎细胞因子的功能参与变应性炎症的发生。
P>Background:Interleukin (IL)-29 is a newly described cytokine that has anti-viral activity, induces tumor cell death and regulates immune function. Whether it plays a role in immune disorders is unclear. This study aims to examine the role of IL-29 in the modulation of immune response under allergic environment.Methods:A group of patients with allergic asthma or/and allergic rhinitis was recruited to this study. Serum samples were collected from the patients in both in-season and out-season; the serum levels of IL-29 were determined by enzyme-linked immunoassay. Cell types of IL-29-producing cells in upper airway mucosa were identified with immune staining and examined by immunohistochemistry and flow cytometry.Results:High serum levels of IL-29 were detected in patients with allergic asthma in in-season, but not in out-season. The majority of IL-29+ cells in upper airway tissue were CD14+ cells. Exposure to specific antigens triggered the release of IL-4 from antigen-specific CD4+ T cells; the released IL-4 activated CD14+ cells to release IL-29; the released IL-29 further triggered the release of IL-6 and tumor necrosis factor from CD4+ T cells.Conclusions:Interleukin-29 is involved in the pathogenesis of allergic inflammation via modulating immune cells' function to release proinflammatory cytokines.