Chlorhexidine preserves dentin bond in vitro
Chlorhexidine preserves dentin bond in vitro
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DOI：
10.1177/154405910708600115
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发表时间：
2007-01-01
影响因子：
7.6
通讯作者：
Tjaderhane, L.
中科院分区：
文献类型：
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作者：
Carrilho, M. R. O.;Carvalho, R. M.;Tjaderhane, L.
混合层完整性的丧失损害树脂-牙本质粘结稳定性。基质金属蛋白酶（MMP）可能是部分负责混合层降解。由于洗必泰抑制基质金属蛋白酶，我们假设洗必泰将减缓树脂牙本质键的损失。将提取的第三磨牙中的I类制剂分成两半。其中一半通常进行修复（蚀刻和冲洗粘合剂/树脂复合物），另一半在修复前酸蚀后用2%洗必泰处理。将标本储存在含/不含蛋白酶抑制剂的人工唾液中。分别在试件制备后即刻和6个月后，在扫描电镜下观察微拉伸粘结强度和破坏模式分布。使用洗必泰，6个月后观察到粘合强度的保存明显更好;储存介质中的蛋白酶抑制剂没有影响。失效分析显示，6个月后，与对照组相比，氯己定混合层的失效率显著降低。总之，这项体外研究表明，洗必泰可能是有用的牙本质粘结强度的保存。
Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens' preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.