Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi
Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi
复制标题
DOI：
10.3390/en7063872
复制
发表时间：
2014-06-01
期刊：
影响因子：
3.2
通讯作者：
Taherzadeh, Mohammad J.
中科院分区：
文献类型：
--
作者：
Ferreira, Jorge A.;Lennartsson, Patrik R.;Taherzadeh, Mohammad J.
每年生产200，000 m（3）乙醇的淀粉基乙醇设施也生产约100，000 m（3）乙醇。200万m3稀酒糟，可用于改善整个工艺。在这项工作中，五个食品级丝状真菌，包括接合菌和四个子囊菌成功地生长在含有9%固体的稀酒糟。用中间脉孢菌培养导致ca. 16 g.L-1生物质，含有56%（w/w）粗蛋白，总固体减少34%，和5 g.L-1额外的乙醇。在工业乙醇生产过程中（200，000 m（3）乙醇/年），这可能导致每年额外生产11，000 m（3）乙醇。在子囊菌中，用曲霉菌培养导致19 g.L-1的生物质含有48%（w/w）的粗蛋白和最高的稀酒糟甘油减少（54%）。用根霉培养产生高达15 g.L-1的生物量，含有55%（w/w）的粗蛋白。废酒糟中的乳酸、甘油和总固形物分别减少了85%、68%和21%。因此，N.特别是，中间体具有通过生产额外的乙醇和高质量生物质来改进乙醇工艺的高潜力，这可以被考虑用于动物饲料应用，例如用于鱼饲料。
A starch-based ethanol facility producing 200,000 m(3) ethanol/year also produces ca. 2 million m(3) thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a Zygomycete and four Ascomycetes were successfully grown in thin stillage containing 9% solids. Cultivation with Neurospora intermedia led to the production of ca. 16 g.L-1 biomass containing 56% (w/w) crude protein, a reduction of 34% of the total solids, and 5 g.L-1 additional ethanol. In an industrial ethanol production process (200,000 m(3) ethanol/year), this can potentially lead to the production of 11,000 m(3) extra ethanol per year. Cultivation with Aspergillus oryzae resulted in 19 g.L-1 biomass containing 48% (w/w) crude protein and the highest reduction of the thin stillage glycerol (54%) among the Ascomycetes. Cultivation with Rhizopus sp. produced up to 15 g.L-1 biomass containing 55% (w/w) crude protein. The spent thin stillage had been reduced up to 85%, 68% and 21% regarding lactic acid, glycerol and total solids, respectively. Therefore, N. intermedia, in particular, has a high potential to improve the ethanol process via production of additional ethanol and high-quality biomass, which can be considered for animal feed applications such as for fish feed.