Establishment of nude mouse transplantable model of a human adenoid cystic carcinoma of the oral floor showing metastasis to the lymph node and lung.
Establishment of nude mouse transplantable model of a human adenoid cystic carcinoma of the oral floor showing metastasis to the lymph node and lung.
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DOI：
10.3892/or.17.1.67
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发表时间：
2007
期刊：
影响因子：
4.2
通讯作者：
S. Hashitani;M. Urade;Yusuke Zushi;Emi Segawa;Shin Okui;K. Sakurai
中科院分区：
文献类型：
--
作者：
S. Hashitani;M. Urade;Yusuke Zushi;Emi Segawa;Shin Okui;K. Sakurai
腺样囊性癌是一种生长缓慢但高度恶性的涎腺肿瘤，具有显著的局部侵袭和肺转移能力。由于没有合适的动物模型，对ACC的确切特征还没有完全了解。我们已经成功地建立了一种新的人类肿瘤细胞系(ACCI)，它来源于口底的ACC，在组织学上显示出筛状模式，并可连续移植到裸鼠体内。该肿瘤在第二代水平自发转移至颈部，组织学特征由ACC转变为未分化癌(ACCIM)。ACCIM皮下移植到裸鼠体内后，可引起高发病率的肺转移。在这项研究中，我们研究了这一有趣的人类ACC转移株的特征。将肿瘤片段移植到裸鼠皮下，每隔1周测量肿瘤生长情况。进行组织学和免疫组织化学检查。结果表明，与ACCI相比，ACCIM的肿瘤生长速度加快，增殖细胞核抗原标记指数升高。移植后5个月，ACCIM小鼠出现多处淋巴结和肺部转移，6个月内全部死亡。这些多发性转移也在舌头原位移植中得到证实。RT-PCR分析表明，ACCIM表达人β-肌动蛋白，表明其来源于人类。从这些发现来看，ACCIM移植到裸鼠体内将为研究ACC的生物学行为提供一个有用的模型。
Adenoid cystic carcinoma (ACC) is a generally slow-growing but highly malignant salivary gland neoplasm with remarkable capacities for local invasion and lung metastasis. The precise characteristics of ACC are not fully understood because there was no suitable animal model. We have successfully established a new human tumor line (ACCI) derived from ACC of the oral floor, which showed a cribriform pattern histologically and serially transplantable into nude mice. This tumor developed spontaneous metastasis to the neck at the second passage level, and the histological feature changed from ACC to undifferentiated carcinoma (ACCIM). ACCIM caused spontaneous metastasis to the lung at high incidence when transplanted subcutaneously in nude mice. In this study, we examined the characteristics of this interesting human ACC metastatic line. Tumor fragments were subcutaneously transplanted into nude mice and tumor growth was measured at 1-week intervals. Histological and immunohistochemical examinations were performed. As a result, the tumor growth rate of ACCIM increased as compared to that of ACCI, and the PCNA labeling index was elevated. Furthermore, ACCIM produced multiple metastases to lymph nodes and lungs 5 months after transplantation, and all mice died within 6 months. These multiple metastases were also confirmed in orthotopic transplantation to the tongue. RT-PCR analysis revealed that ACCIM expressed human beta-actin, indicating its human origin. From these findings, ACCIM transplanted into nude mice would provide a useful model for investigating the biological behaviour of ACC.