Engineered gold nanoparticles as multicolor labels for simultaneous multi- mycotoxin detection on the immunochromatographic test strip nanosensor
Engineered gold nanoparticles as multicolor labels for simultaneous multi- mycotoxin detection on the immunochromatographic test strip nanosensor
复制标题
工程金纳米粒子作为多色标记，用于在免疫色谱测试条纳米传感器上同时检测多种霉菌毒素
DOI：
10.1016/j.snb.2020.128107
复制
发表时间：
2020-07-15
影响因子：
8.4
通讯作者：
Xiong, Yonghua
中科院分区：
文献类型：
--
作者：
Wu, Yuhao;Zhou, Yaofeng;Xiong, Yonghua
Herein, we report a multicolor immunochromatographic test strip (ICTS) nanosensor for the rapid and simultaneous visual detection of fumonisin B-1 (FB1), zearalenone (ZEN), ochratoxin A (OTA), and aflatoxin B-1 (AFB(1)). Four colored AuNPs, including gold nanospheres (red), gold nanocacti (purple), gold nanoflowers (blue), and hyperbranched Au plasmonic blackbodies (black), were synthesized and functionalized with the corresponding antibodies to target each analyte. The developed multicolor platform improved bare-eye-based visual detection ability with cut-off values of 120, 60, 2.5, and 5 ng/mL for FB1, ZEN, OTA, and AFB(1), respectively. The limits of detection for FB1, ZEN, OTA, and AFB(1) were calculated as 3.27, 0.70, 0.10, and 0.06 ng/mL, respectively, and the average recoveries of the four mycotoxins ranged from 82.36% to 116.23% with a small coefficient of variation (below 12.98%). Accuracy, stability, reliability, and practicability were demonstrated by analyzing artificially and naturally contaminated corn samples. In summary, the designed multicolor ICTS nanosensor can serve as an alternative rapid diagnostic platform enabling the sensitive, simultaneous, and on-site screening detection of mycotoxins and other analytes.
