Effective adsorption and separation of lysozyme with PAA-modified Fe3O4@silica core/shell microspheres.
Effective adsorption and separation of lysozyme with PAA-modified Fe3O4@silica core/shell microspheres.
复制标题
DOI：
10.1016/j.jcis.2009.02.061
复制
发表时间：
2009-08
影响因子：
9.9
通讯作者：
Dandan Shao;Keke Xu;Xiaojie Song;Jianhua Hu;Wuli Yang;Changchun Wang
中科院分区：
文献类型：
--
作者：
Dandan Shao;Keke Xu;Xiaojie Song;Jianhua Hu;Wuli Yang;Changchun Wang
采用溶胶-凝胶法制备了亚微米级Fe3O4@silica核/壳微球。通过包覆惰性硅壳和调整厚度，磁性微球在水中的分散性大大提高。所得Fe3O4@silica微球具有超顺磁性，饱和磁化强度在34.3 ~ 47.3emu/g之间可控。所有制备的磁性复合微球都具有快速的磁响应性。采用聚丙烯酸修饰Fe3O4@silica微球对溶菌酶进行选择性吸附分离，实验结果表明，其最大结合容量约为127mg/g。用聚丙烯酸（PAA）柔性链对Fe3O4@silica进行表面改性后，在相同pH条件下，Fe3O4@silica-g-PAA微球对溶菌酶的结合能力是纯Fe3O4@silica微球的22倍。
Submicron-size Fe3O4@silica core/shell microspheres were prepared by sol–gel method. Through coating inert silica shell and adjusting the thickness, the dispersibility of magnetic microspheres in water was greatly improved. The resultant Fe3O4@silica microspheres with controllable saturation magnetization between 34.3emu/g and 47.3emu/g were superparamagnetic. All as-prepared magnetic composite microspheres possessed fleetly magnetic responsivity. The PAA-modified Fe3O4@silica microspheres were used for selective adsorption and separation of lysozyme, and the experimental results showed that the maximum binding capacity is about 127mg/g. After surface modification of Fe3O4@silica with polyacrylic acid (PAA) flexible chains, the binding capacity of Fe3O4@silica-g-PAA microspheres for lysozyme were 22 times as much as that of pure Fe3O4@silica microspheres at the same pH condition.