Regional organization of pre-pacemaker cells in the cardiac primordia of the early chick embryo.
Regional organization of pre-pacemaker cells in the cardiac primordia of the early chick embryo.
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早期鸡胚心脏原基中前起搏器细胞的区域组织。
DOI：
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发表时间：
1963
期刊：
影响因子：
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通讯作者：
R. Dehaan
中科院分区：
文献类型：
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作者：
R. Dehaan
有充分的证据表明，鸡胚心脏原基的细胞在心脏出现任何组织学或形态学迹象之前很久就已经经历了实质性的化学分化。Rawles（1943）将头突期胚胎（第5期，Hamburger-Hamilton系列，1951）中潜在的心脏中胚层定位于一对侧向心脏形成区。正是在这个时候，Ebert等（1953，1955）已经表明，相同的两个区域对心脏肌动蛋白和肌球蛋白的抗原结合基团呈阳性反应。DufFey和Ebert（1957）利用Spratt（1950）关于胚胎心脏与其他胚胎组织相比对氟离子敏感性的证明，还表明氟离子对第5阶段胚胎的细胞毒性作用几乎完全限于心脏形成区域的细胞。这些心前中胚层的区域在延时电影中可以看到，它们是通过内胚层形成的黑色浓缩区（DeHaan，1961 a，1963 a）。
There is ample evidence that the cells of the cardiac primordia in the chick embryo have undergone substantial chemodifferentiation long before any histological or morphological sign of the heart appears. Rawles (1943) has localized the position of the potential heart mesoderm in the head-process stage embryo (Stage 5, Hamburger-Hamilton series, 1951) to a pair of lateral heartforming regions. It is at this time that Ebert et al. (1953, 1955) have shown that the same two areas react positively for antigenic combining groups of cardiac actin and myosin. Taking advantage of Spratt9s demonstration (1950) of the sensitivity to fluoride ion of embryonic heart, as compared with other embryonic tissues, DufFey & Ebert (1957) have also shown that the cytotoxic effect of this ion on the stage 5 embryo is limited almost entirely to the cells in the heart-forming regions. These regions of precardiac mesoderm can be seen on time-lapse motion picture films as dark condensed areas silhouetted through the endoderm (DeHaan, 1961 a , 1963 a ).