Classification of male factor infertility relevant to in-vitro fertilization insemination strategies using mannose ligands, acrosome status and anti-cytoskeletal antibodies.
Classification of male factor infertility relevant to in-vitro fertilization insemination strategies using mannose ligands, acrosome status and anti-cytoskeletal antibodies.
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使用甘露糖配体、顶体状态和抗细胞骨架抗体对与体外受精授精策略相关的男性不育因素进行分类。
DOI：
10.1093/oxfordjournals.humrep.a019516
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Hershlag,A
中科院分区：
文献类型：
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作者：
Benoff,S;Barcia,M;Hurley,IR;Cooper,GW;Mandel,FS;Heyner,S;Garside,WT;Gilbert,BR;Hershlag,A
多价甘露糖配体在游离甘露糖存在下充当透明带激动剂，在体外获能后快速诱导来自可育供体的有能力的活动人类精子的顶体胞吐作用。对异硫氰酸荧光素标记的甘露糖化白蛋白和识别甘露糖受体和其他相关精子膜蛋白的特异性抗血清的结合模式进行定量，以及获能后诱导顶体胞吐作用的发生率，使我们能够识别三类男性不育症。 1 类雄性的精子精液参数正常，其精子的精子胆固醇值升高，并且在标准短期孵化后无法在体外使卵母细胞受精。这些精子不结合甘露糖配体，也不表现出自发或诱导的顶体反应，但从精子中去除胆固醇的治疗（例如在甾醇受体存在下延长孵育）赋予受精的能力。胆固醇加载和卸载实验已经证明了1类男性不育症中精子膜特性的可逆特征。 2 类雄性精液中的精子外观正常，在孵化时表达甘露糖配体受体，但无法对甘露糖处理做出一些反应。有趣的是，本研究中发现的所有 2 类男性都患有临床精索静脉曲张。第 3 类男性的精液可能是正常精子或畸形精子，在某些情况下，在没有临床精索静脉曲张的情况下，锥形精子的比例很高。来自第 3 类男性的精子缺乏完整膜蛋白超家族，通过氨基酸序列分析和抗肌球蛋白抗体反应性鉴定，其细胞质尾部具有肌球蛋白马达。它们的精子不表达甘露糖配体受体或进行诱导的顶体反应。 2 类和 3 类精液的受精只能通过显微操作程序实现。这些发现说明了不孕症分类基础研究的实际应用。
Polyvalent mannose ligands in the presence of free mannose act as zona pellucida agonists which rapidly induce acrosome exocytosis in competent motile human sperm from fertile donors following in-vitro capacitation. Quantification of the binding patterns of fluorescein isothiocyanate labelled mannosylated albumins and of specific antisera which recognize mannose receptors and other related integral sperm membrane proteins as well as the incidence of induced acrosome exocytosis after capacitation has allowed us to identify three categories of male infertility. Category 1 males have normozoospermic semen parameters, their spermatozoa have elevated sperm cholesterol values and fail to fertilize oocytesin vitroafter standard short-term incubations. These spermatozoa do not bind mannose ligands and do not show spontaneous or induced acrosome reactions, but treatments to remove cholesterol from the spermatozoa (e.g. prolonged incubation in the presence of sterol acceptors) confer the ability to fertilize. Cholesterol loading and unloading experiments have demonstrated the reversible character of sperm membrane properties in category 1 male infertility. Category 2 males have normal-appearing spermatozoa in semen which express mannose ligand receptors on incubation, but fail to undergo acro some reactions in response to mannose treatment. Interestingly, all category 2 males identified in this study have clinical varicocele. Category 3 males have semen which may be normozoospermic or teratozoospermic with, in some cases, high percentages of tapering spermatozoa in the absence of clinical varicocele. Spermatozoa from category 3 men are deficient in a superfamily of integral membrane proteins whose cytoplasmic tails have myosin motors as identified by amino acid sequence analysis and anti-myosin antibody reactivity. Their spermatozoa do not express mannose ligand receptors or undergo induced acrosome reactions. Fertilization with category 2 and 3 semen is only achieved by micromanipulation procedures. These findings illustrate the practical application of basic research for infertility classification.