Anopheles culicifacies Y-chromosome dimorphism indicates sibling species (B and E) with different malaria vector potential in Sri Lanka
Anopheles culicifacies Y-chromosome dimorphism indicates sibling species (B and E) with different malaria vector potential in Sri Lanka
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DOI：
10.1046/j.1365-2915.2000.00264.x
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发表时间：
2000-12-01
影响因子：
1.9
通讯作者：
Ramasamy, MS
中科院分区：
文献类型：
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作者：
Surendran, SN;Abhayawardana, TA;Ramasamy, MS
在斯里兰卡，疟疾主要由库氏按蚊传播（双翅目：库氏按蚊科）。在印度，这个名义分类群包括兄弟种A、B、C、D和E，以其染色体形态区分。B种(由多烯染色体序列Xab鉴定，2g（1) + h(1)）不像其他亚种那样是有效的疟疾媒介。印度的文化相复合体。An的所有标本。来自斯里兰卡的culicifacies s.l.具有Xab， 2g(1) + h(1)多烯，尽管它们具有重要的媒介地位，但以前被解释为B种。最近，在斯里兰卡和印度大陆之间的Rameshwaram岛（印度泰米尔纳德邦）发现了E种，B种和E种都是同域的。物种B和E共享多烯序列Xab， 2g(1) + h(1)，但不同之处在于有丝分裂的y染色体在物种B中是单中心的，在物种E中是亚亚中心的，后者可能是间日疟疾的载体。从1999年5月到2000年1月，我们对安氏雄性后代的y染色体进行了检测。培养皿相Xab， 2g(1) + h(1)雌性，采集自斯里兰卡不同疟疾流行地区的牛饵：Badulla、Monaragala、Puttalam和亭可马里。从42/83个家系中获得了可读质量的核型，总体比例为24%的多中心y染色体携带者和76%的亚多中心y染色体携带者，这两种类型在至少3/4的取样地点具有同域性。通过与Rameshwaram岛的情况类比，我们将这些观察结果解释为证明了An的两个成员的广泛存在。它们的核型与B和E种相容，后者占主导地位，具有更大的媒介潜力。
In Sri Lanka, malaria is transmitted mainly by Anopheles culicifacies Giles sensu lato (Diptera: Culicidae). In India, this nominal taxon comprises sibling species A, B, C, D and E, distinguished by their chromosome morphology. Species B (identified by polytene chromosome sequence Xab, 2g(1) + h(1)) is not such an efficient vector of malaria as other members of the An. culicifacies complex in India. All specimens of An. culicifacies s.l. examined from Sri Lanka possess Xab, 2g(1) + h(1) polytenes, previously interpreted as species B, despite their important vector status. Recently, species E was described from Rameshwaram Island (Tamil Nadu, India) between Sri Lanka and the Indian mainland, where both species B and E are sympatric. Species B and E share polytene sequence Xab, 2g(1) + h(1) but differ by the mitotic Y-chromosome being acrocentric in species B, submetacentric in species E, the latter implicated as vector of vivax malaria. From May 1999 to January 2000, we surveyed Y-chromosomes of male progeny from An. culicifacies Xab, 2g(1) + h(1) females collected from cattle bait in diverse malarious districts of Sri Lanka: Badulla, Monaragala, Puttalam and Trincomalee. Karyotypes of readable quality were obtained from 42/83 families examined, with overall proportions 24% acrocentric and 76% submetacentric Y-chromosome carriers, both types being sympatric in at least 3/4 localities sampled. By analogy with the situation on Rameshwaram Island, we interpret these observations to demonstrate widespread presence of two members of the An. culicifacies complex in Sri Lanka, their karyotypes being compatible with species B and E, the latter predominant and having greater vector potential.