Isolation and characterization of mutants of Arabidopsis thaliana with increased resistance to growth inhibition by indoleacetic acid-amino acid conjugates
Isolation and characterization of mutants of Arabidopsis thaliana with increased resistance to growth inhibition by indoleacetic acid-amino acid conjugates
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DOI：
10.1104/pp.112.2.735
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发表时间：
1996-10-01
期刊：
影响因子：
7.4
通讯作者：
Town, CD
中科院分区：
文献类型：
--
作者：
Campanella, JJ;LudwigMueller, J;Town, CD
从拟南芥（Arabidopsis thaliana）中分离到两个抗吲哚乙酸（IAA）-苯丙氨酸生长抑制的突变体。这两种突变体的2- 3倍以上的抗性比野生型抑制IAA-苯丙氨酸，IAA-丙氨酸，和IAA-甘氨酸在根生长测定。突变体icr 1（而不是icr 2）也显示出一定的抗性IAA-天冬氨酸。使用H-3-标记的IAA-苯丙氨酸的研究表明，icr 1从培养基中摄取缀合物与野生型相同，icr 2中减少约25%。在突变体和它们的野生型亲本之间检测到外源缀合物的水解没有差异。从[H-3] IAA-苯丙氨酸释放的IAA没有显著的代谢，而外源性[H-3]IAA被迅速代谢成两种比IAA极性大得多的未鉴定产物。ICR 1和ICR 2之间的交叉分析表明，这些突变是在不同的位点，其效果是加性的，初步定位数据表明，ICR 1和ICR 2分别位于V染色体的顶部和底部。
Two mutants of Arabidopsis thaliana that are resistant to growth inhibition by indole-3-acetic acid (IAA)-phenylalanine have been isolated. Both mutants were 2- to 3-fold more resistant than wild type to inhibition by IAA-phenylalanine, IAA-alanine, and IAA-glycine in root growth assays. The mutant icr1 (but not icr2) also shows some resistance to IAA-aspartate. Studies using H-3-labeled IAA-phenylalanine showed that the uptake of conjugate from the medium by icr1 was the same as wild type and was reduced by about 25% in icr2. No differences in hydrolysis of the exogenous conjugate were detected between the mutants and their wild-type parents. There was no significant metabolism of the IAA released from the [H-3]IAA-phenylalanine, whereas exogenous [H-3]IAA was rapidly metabolized to two unidentified products considerably more polar than IAA. Analysis of a cross between icr1 and icr2 indicated that these mutations were at distinct loci and that their effects were additive, and preliminary mapping data indicated that icr1 and icr2 were located at the top and bottom of chromosome V, respectively.