Heat shock induction of heme oxygenase mRNA in human Hep 3B hepatoma cells.
Heat shock induction of heme oxygenase mRNA in human Hep 3B hepatoma cells.
复制标题
人 Hep 3B 肝癌细胞中血红素加氧酶 mRNA 的热激诱导。
DOI：
10.1016/0006-291x(89)91089-9
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Kappas,A
中科院分区：
文献类型：
--
作者：
Mitani,K;Fujita,H;Sassa,S;Kappas,A
热休克处理人Hep 3B肝癌细胞导致微粒体血红素加氧酶mRNA的诱导。在43.5°C下处理细胞60 min，观察到血红素加氧酶mRNA的最大诱导（5→7倍）。同时用放线菌素D或放线菌酮处理细胞，可阻断血红素加氧酶mRNA的热介导诱导。与Hep 3B细胞相反，另一种人肝癌细胞系Hep G2的细胞通过热处理几乎不显示血红素加氧酶mRNA的诱导。这些结果表明，热休克处理诱导血红素加氧酶mRNA在某些人肝癌细胞，但不是在其他人。
Heat shock treatment of human Hep 3B hepatoma cells led to the induction of mRNA for microsomal heme oxygenase. The maximum induction of heme oxygenase mRNA (5→7-fold) was observed with treatment of cells at 43.5°C, for 60 min. The heat-mediated induction of heme oxygenase mRNA was blocked by simultaneous treatment of cells with actinomycin D or cycloheximide. In contrast to Hep 3B cells, cells of another human hepatoma line, Hep G2, showed little induction of heme oxygenase mRNA by heat treatment. These findings suggest that heat shock treatment induces heme oxygenase mRNA in certain human hepatoma cells, but not in others.