Mapping the developing retinotectal projection in frog tadpoles by a double label autoradiographic technique
Mapping the developing retinotectal projection in frog tadpoles by a double label autoradiographic technique
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通过双标签放射自显影技术绘制青蛙蝌蚪发育中的视网膜顶盖投影
DOI：
10.1016/0006-8993(77)90379-1
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
M. Jacobson
中科院分区：
文献类型：
--
作者：
M. Jacobson
用眼内注射[~ 3 H]脯氨酸和腹腔注射[~ 3 H]胸苷处理45-59期的Xenopus蝌蚪，存活1-2天或16-37天后处死，进行放射自显影。在所有情况下，都有一个有规律的标签模式。在视网膜中，脯氨酸标记的细胞总是中央的胸腺嘧啶标记的细胞核，并在长期的幸存者有一个急剧的过渡之间的细胞占据中央标记的视网膜和周围的未标记的细胞在视网膜周边。在顶盖，未标记的视神经轴突被限制在一个rostrolateral区，这是接壤的区域caudomedially标记顶盖细胞核，并在长期的幸存者有一个完全未标记的区域在tectum的尾内侧边缘。标记的轴突的顶盖区的大小是没有显着不同的长期幸存者在同一发展阶段注射的短期幸存者。标记的视神经轴突仍然接近注射前存在的顶盖细胞，并且在注射后的存活期内没有移动到添加到顶盖的细胞区中。
Tadpoles ofXenopusat developmental stages 45–59 were given an intraocular injection of [3H]proline and an intraperitoneal injection of [3H]thymidine, killed either after a short (1–2 day) or long (16–37 day) survival period, and processed for autoradiography. In all cases, there was a regular pattern of labelling. In the retina, the proline-labelled cells were always central to the thymidine-labelled cell nuclei, and in the long survivors there was a sharp transition between the cells occupying the central labelled retina and the surrounding unlabelled cells at the retinal periphery. In the tectum, unlabelled optic axons were confined to a rostrolateral zone which was bordered caudomedially by a zone of labelled tectal cell nuclei, and in the long survivors there was a completely unlabelled zone at the caudomedial margin of the tectum. The size of the tectal zone of labelled axons was not significantly different in the long survivors from that in short survivors injected at the same stage of development. Labelled optic axons remained close to the tectal cells that were present before the time of injection and did not move later into the zone of cells added to the tectum in the survival period after the injections.