VASCULAR-PERMEABILITY FACTOR (VASCULAR ENDOTHELIAL GROWTH-FACTOR) GENE IS EXPRESSED DIFFERENTIALLY IN NORMAL-TISSUES, MACROPHAGES, AND TUMORS
VASCULAR-PERMEABILITY FACTOR (VASCULAR ENDOTHELIAL GROWTH-FACTOR) GENE IS EXPRESSED DIFFERENTIALLY IN NORMAL-TISSUES, MACROPHAGES, AND TUMORS
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DOI：
10.1091/mbc.3.2.211
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发表时间：
1992-02-01
影响因子：
3.3
通讯作者：
SENGER, DR
中科院分区：
文献类型：
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作者：
BERSE, B;BROWN, LF;SENGER, DR
血管通透性因子（VPF），也称为血管内皮生长因子（VEGF），增加微血管通透性，是内皮细胞的特异性有丝分裂原。VPF/VEGF的表达以前被证明在各种肿瘤细胞中，在培养的垂体源性细胞，并在黄体。在这里，我们提出的证据，通过北方分析和原位杂交，VPF/VEGF基因表达在许多成人器官，包括肺，肾，肾上腺，心脏，肝脏，胃粘膜，以及在引发的腹腔巨噬细胞。VPF/VEGF转录水平最高的是肺泡上皮细胞，肾小球和肾上腺皮质，心肌细胞。VPF/VEGF mRNA在这些组织中的显著性表明VPF/VEGF在调节基线微血管通透性中可能起作用，这对于组织营养和废物清除至关重要。我们还证明了特别高的VPF/VEGF mRNA水平在几个人类肿瘤，它可能参与促进肿瘤血管生成和基质的产生，既作为一种内皮细胞有丝分裂原，并间接通过其渗透性增强作用，导致临时纤维蛋白凝胶基质的沉积。
Vascular permeability factor (VPF), also known as vascular endothelial growth factor (VEGF), increases microvascular permeability and is a specific mitogen for endothelial cells. Expression of VPF/VEGF previously was demonstrated in a variety of tumor cells, in cultures of pituitary-derived cells, and in corpus luteum. Here we present evidence, by Northern analysis and in situ hybridization, that the VPF/VEGF gene is expressed in many adult organs, including lung, kidney, adrenal gland, heart, liver, and stomach mucosa, as well as in elicited peritoneal macrophages. The highest levels of VPF/VEGF transcripts were found in epithelial cells of lung alveoli, renal glomeruli and adrenal cortex, and in cardiac myocytes. The prominence of VPF/VEGF mRNA in these tissues suggests a possible role for VPF/VEGF in regulating baseline microvascular permeability, which is essential for tissue nutrition and waste removal. We also demonstrate particularly high VPF/VEGF mRNA levels in several human tumors, where it may be involved in promoting tumor angiogenesis and stroma generation, both as an endothelial cell mitogen and indirectly by its permeability enhancing effect that leads to the deposition of a provisional fibrin gel matrix.