Evaluation of myocardial viability during simple cold storage with the use of electrical properties in broad frequencies.
Evaluation of myocardial viability during simple cold storage with the use of electrical properties in broad frequencies.
复制标题
利用宽频率的电特性评估简单冷藏期间的心肌活力。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
S. Murakawa
中科院分区：
文献类型：
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作者：
M. Ishikawa;H. Hirose;E. Sasaki;M. Bando;Y. Mori;S. Murakawa
背景
这项研究的目的是确定是否可以通过保存心脏的电特性变化来监测简单冷藏期间的心肌存活情况。
方法
12只麻醉犬进行快速心脏摘除，保存在4℃的简易冷藏室中。根据保存液的不同分为两组。S组和U组分别保存在生理盐水和威斯康星大学溶液中。用阻抗仪连续测量保存心脏的电学性质，分析介电频谱中的损耗正切(Tan Delta)。取心肌标本，用高效液相色谱法测定心肌组织中三磷酸腺苷的含量，并测定心肌含水量。我们研究了心肌腺苷三磷酸、心肌水分含量和Tan增量之间的相关性。
结果
两组Tan delta值的最大值对应的频率为5~10 kHz，保存过程中两组的Tan delta值均逐渐减小。U组心肌组织中三磷酸腺苷含量明显高于S组。保存过程中测得的三磷酸腺苷和最大Tan增量与缺血前的比值以三磷酸腺苷百分比和最大Tan增量百分比表示。两组保存期间心肌组织三磷酸腺苷百分比与最大TAN增量百分比密切相关。S组心肌水含量在保存过程中增加，而U组无明显增加。保存过程中心肌水含量与最大Tan增量百分比之间无相关性。Tan-Delta函数用于表征生物组织的电学特性，并与组织的缺血性组织学损伤明显相关。
结论
因此，测量保存心脏的宽频电学特性可能是一种可行的微创方法来监测移植物的存活率。
BACKGROUND
The purpose of this study ws to determine whether myocardial viability during simple cold storage can be monitored from alteration of electrical properties of the preserved heart.
METHODS
Twelve anesthetized dogs underwent rapid cardiac extirpation and were preserved in simple cold storage ( 4 degree C). They were divided into two groups according to preservation solutions. Groups S and U were preserved in saline and University of Wisconsin Solutions, respectively. The loss-tangent (Tan delta) in dielectric spectroscopy was analyzed on serial measurements of electrical properties of the preserved heart with an electrical impedance meter. Myocardial specimens were taken for myocardial adenosine triphosphate measurement with high-performance liquid chromatography and myocardial water content. We investigated the correlations among myocardial adenosine triphosphate, myocardial water content, and Tan delta.
RESULTS
The maximum value of Tan delta corresponds to a frequency fT which ranged from 5 to 10 kHz in both groups and maximum Tan delta gradually decreased during preservation in both groups. Myocardial adenosine triphosphate in group U was significantly higher than that in group S. The ratio of measured adenosine triphosphate and maximum Tan delta to the preischemic values during preservation were expressed as percentage of adenosine triphosphate and percentage of maximum Tan delta. There was close correlation between myocardial percentage of adenosine triphosphate and percentage of maximum Tan delta during preservation in both groups. Myocardial water content in group S increased during preservation but did not increase in group U. There was no correlation between myocardial water content and percentage of maximum Tan delta during preservation. Tan delta function were used to characterize the electrical properties of biologic tissue and were clearly associated with ischemic histologic damage of tissues.
CONCLUSIONS
Therefore measurement of electrical properties in broad frequencies of the preserved heart may be feasible as a monitor of graft viability in a minimally invasive method.