Characterization, expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge
Characterization, expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge
复制标题
DOI：
10.1007/s11103-009-9596-5
复制
发表时间：
2010-05-01
影响因子：
5.1
通讯作者：
Anderson, James
中科院分区：
文献类型：
--
作者：
Horvath, David P.;Sung, Sibum;Anderson, James
休眠相关的MADS-BOX（DAM）基因与拟南芥的AGAMOUS-LIKE 24和SHORT VEGETIVE PHASE基因相关，并且在几种多年生植物芽中与内休眠诱导和解除协同地差异调节。DAM基因首次被证明直接影响桃的内休眠，其中一系列DAM基因的缺失导致内休眠诱导的丧失。我们已经从模式多年生杂草多叶大戟中克隆并鉴定了几个MADS盒基因。多叶大戟DAM基因优先在茎尖和芽中表达，以响应低温和日长的方式相对于由各种环境条件诱导的内休眠水平。一个DAM基因在拟南芥中的过表达延迟开花。此外，我们表明，至少有一个DAM基因的差异调节染色质重塑。DAM基因启动子之间的比较杨树和多叶大戟已经确定了几个保守的序列，可能是重要的表达模式，在响应休眠诱导刺激。
DORMANCY ASSOCIATED MADS-BOX (DAM) genes are related to AGAMOUS-LIKE 24 and SHORT VEGETATIVE PHASE genes of arabidopsis and are differentially regulated coordinately with endodormancy induction and release in buds of several perennial plant species. DAM genes were first shown to directly impact endodormancy in peach where a deletion of a series of DAM resulted in loss of endodormancy induction. We have cloned and characterized several MADS box genes from the model perennial weed leafy spurge. Leafy spurge DAM genes are preferentially expressed in shoot tips and buds in response to cold temperatures and day length in a manner that is relative to the level of endodormancy induced by various environmental conditions. Over-expression of one DAM gene in arabidopsis delays flowering. Additionally, we show that at least one DAM gene is differentially regulated by chromatin remodeling. Comparisons of the DAM gene promoters between poplar and leafy spurge have identified several conserved sequences that may be important for their expression patterns in response to dormancy-inducing stimuli.