Cell cycle regulation of the c-Myc transcriptional activation domain
Cell cycle regulation of the c-Myc transcriptional activation domain
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DOI：
10.1128/mcb.13.7.4125-4136.1993
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发表时间：
1993-07
影响因子：
5.3
通讯作者：
A. Seth;Shashi Gupta;Roger J. Davis
中科院分区：
文献类型：
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作者：
A. Seth;Shashi Gupta;Roger J. Davis
c-myc基因的产物（c-Myc）是一种序列特异性DNA结合蛋白，先前已证明是细胞周期进程所需的。在这里，我们报告的c-Myc DNA结合位点赋予中国仓鼠卵巢细胞的报告基因的细胞周期调控。所观察到的反式激活是双相的，在G1中有一个小的增加，在细胞周期的S-到-G2/M过渡期间有显著的增加。这种反式激活潜力的细胞周期调节部分是由于c-Myc反式激活结构域的调节磷酸化。总之，这些数据表明，c-Myc可能在细胞通过细胞周期的G1和G2期的进展中具有重要作用。
The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.