Long-term culture of human antigen-specific cytotoxic T-cell lines.
Long-term culture of human antigen-specific cytotoxic T-cell lines.
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DOI：
10.1084/jem.148.4.1093
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发表时间：
1978-10-01
期刊：
影响因子：
--
通讯作者：
Smith KA
中科院分区：
文献类型：
--
作者：
Gillis S;Baker PE;Ruscetti FW;Smith KA
建立了人细胞毒性T细胞系（H-CTLL）的长期培养物。H-CTLL细胞严格依赖于由植物血凝素刺激的正常人外周血淋巴细胞产生的T细胞生长因子（TCGF）的生长。H-CTLL细胞保持在TCGF依赖性指数增殖培养超过4个月，在此期间，他们继续介导刺激抗原特异性细胞毒性，通过4小时51铬释放测定。从冷冻保存的储备物中回收并在长期培养中重新建立的H-CTLL细胞表现出类似的高水平的抗原特异性细胞毒性。H-CTLL细胞E玫瑰花结阳性率为95- 100%，表达正常的T和Ia样细胞表面标志。在长期培养中维持分化的抗原特异性T效应细胞的能力可能为研究T细胞介导的免疫机制和调节提供新的手段。
Long-term cultures of human cytotoxic T-cell lines (H-CTLL) were established. H-CTLL cells were strictly dependent on growth upon a T- cell growth factor (TCGF) produced by phytohemagglutinin-stimulated normal human peripheral blood lymphocytes. H-CTLL cells were maintained in TCGF-dependent exponential proliferative culture for over 4 mo during which time they continued to mediate stimulator antigen-specific cytotoxicity as measured by a 4-h 51Cr-release assay. H-CTLL cells recovered from cryopreserved stocks and re-established in long-term culture demonstrated similar high levels of antigen-specific cytotoxicity. H-CTLL cells were 95--100% E-rosette positive and expressed normal T and Ia-like cell surface markers. The ability to sustain differentiated antigen-specific T-effector cells in long-term culture may provide a new means for the study of both the mechanism and regulation of T-cell-mediated immunity.