CRITICAL-EVALUATION OF CYTOSOLIC CALCIUM DETERMINATION IN RESTING MUSCLE-FIBERS FROM NORMAL AND DYSTROPHIC (MDX) MICE
CRITICAL-EVALUATION OF CYTOSOLIC CALCIUM DETERMINATION IN RESTING MUSCLE-FIBERS FROM NORMAL AND DYSTROPHIC (MDX) MICE
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DOI：
10.1016/0143-4160(93)90006-r
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发表时间：
1993-06-01
期刊：
影响因子：
4
通讯作者：
GILLIS, JM
中科院分区：
文献类型：
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作者：
GAILLY, P;BOLAND, B;GILLIS, JM
用荧光探针Fura-2/AM测定比目鱼肌和离体趾短屈肌纤维胞浆游离钙浓度。这需要精确的校准，因此，每个校准参数进行了研究。还检查了染料浓度对钙测量的影响。这种精确的校准技术用于比较营养不良（mdx）和对照（C57）小鼠静息制剂中的绝对游离钙浓度。我们发现，染料的行为在C57和mdx肌肉中并不相似。由于这个原因，我们没有证实以前的结果，胞质游离钙浓度增加mdx肌肉。
The fluorescent probe Fura-2/AM was used to determine cytosolic free calcium concentration in soleus muscle and in Isolated flexor digitorum brevis fibres. This required a precise calibration; therefore, each calibration parameter was studied in situ. The influence of the dye concentration on calcium measurements was also examined. This precise calibration technique was used to compare absolute free calcium concentration in resting preparations from dystrophic (mdx) and control (C57) mice. We showed that the behavior of the dye was not similar in C57 and in mdx muscles. For this reason, we did not confirm the previous results that cytosolic free calcium concentration is increased in mdx muscles.