Ultrasound mediated transdermal insulin delivery in pigs using a lightweight transducer
Ultrasound mediated transdermal insulin delivery in pigs using a lightweight transducer
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DOI：
10.1007/s11095-007-9306-4
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发表时间：
2007-07-01
影响因子：
3.7
通讯作者：
Smith, Nadine Barrie
中科院分区：
文献类型：
--
作者：
Park, E. J.;Werner, Jacob;Smith, Nadine Barrie
目的。在以往的研究中，超声介导的经皮给药作为一种无创给药方法显示出很好的潜力。为了展望未来的人类应用，本研究旨在确定轻型钹式传感器阵列作为大型猪无创透皮胰岛素输送实用装置的可行性。材料与方法。6头约克郡猪（100-140磅）被分成两组。作为对照组（n=3），第一组不接受任何胰岛素超声暴露。第二组（n=3）接受超声和胰岛素治疗，频率为20 kHz， I-sptp =100 mW/cm(2)，占空比为20%，持续60分钟。麻醉后猪侧卧，将胰岛素超声换能器置于猪腋窝区域。在开始和每15分钟至90分钟，使用血糖监测系统测定血糖水平。为了比较个体动物的结果，将血糖水平的变化归一化到实验开始时每只动物的初始血糖值。虽然每只动物的初始葡萄糖水平不同，但6只动物的平均值和标准误差为146 +/- 13 mg/dl。对于对照组，在90分钟的实验中，与初始基线相比，血糖水平上升到31 +/- 21 mg/dl。超声治疗组血糖水平在60 min降至-72 +/- 5 mg/dl (p< 0.05)， 90 min降至-91 +/- 23 mg/dl （p< 0.05）
Purpose. In previous studies, ultrasound mediated transdermal drug delivery has shown a promising potential as a method for noninvasive drug administration. For prospective future human application, this study was designed to determine the feasibility of lightweight cymbal transducer array as a practical device for noninvasive transdermal insulin delivery in large pigs.Materials and Methods. Six Yorkshire pigs (100-140 lbs) were divided into two groups. As the control (n=3), the first group did not receive any ultrasound exposure with the insulin. The second group (n=3) was treated with ultrasound and insulin at 20 kHz with an I-sptp =100 mW/cm(2) at a 20% duty cycle for 60 min. With the pigs in lateral recumbency after anesthesia, the ultrasound transducer with insulin was placed on the axillary area of the pig. At the beginning and every 15 min up to 90 min, the blood glucose level was determined using a glucose monitoring system. To compare the results of individual animals, the change of blood glucose level was normalized to each animal's initial glucose value at the start of the experiment.Results. Although each animal had a different initial glucose level, the mean and standard error for the six animals was 146 +/- 13 mg/dl. For the control group, the blood glucose level increased to 31 +/- 21 mg/dl compared to the initial baseline over the 90 min experiment. However for the ultrasound with insulin treated group, the glucose level decreased to -72 +/- 5 mg/dl at 60 min (p< 0.05) and continued to decrease to -91 +/- 23 mg/dl in 90 min (p