Use of the haemopoietic progenitor cell count of the Sysmex SE-9500 to refine apheresis timing of peripheral blood stem cells
Use of the haemopoietic progenitor cell count of the Sysmex SE-9500 to refine apheresis timing of peripheral blood stem cells
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DOI：
10.1046/j.1365-2141.1999.01584.x
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发表时间：
1999-08-01
影响因子：
6.5
通讯作者：
Machin, SJ
中科院分区：
文献类型：
--
作者：
Pollard, Y;Watts, MJ;Machin, SJ
Sysmex SE-9500 自动细胞计数器可对称为“造血祖细胞”(HPC) 的未成熟细胞进行估计。本研究的目的是将 HPC 计数与动员外周血中的 CD34(+) 细胞水平联系起来，并确定 HPC 计数是否有助于预测 CD34(+) 细胞的单采率。对来自 67 名接受祖细胞动员的患者的 114 个样本进行了研究。在稳定状态下检测不到 HPC 细胞。在血浆分离术当天，HPC 和 CD34 计数呈弱相关，中位 KPC 计数比 CD34(+) 细胞计数高 2.3 倍。 HPC 计数不包括 CD34(+) 细胞，因为 CD34(+) 细胞的免疫磁耗竭并没有显着减少 HPC 计数。 CD34(+) 细胞计数预测当天的单采产量 (r=0.773)，HPC 计数也是如此 (r=0.623)。防止不必要的收获、同时最大限度地减少错过充分收获的风险以及最大限度地减少实验室调查的最佳策略是筛选所有样品的 HPC 并将 CD34(+) 细胞测量限制为具有 HPC 计数的样品
The Sysmex SE-9500 automated cell counter provides an estimate of immature cells referred to as 'haemopoietic progenitor cells' (HPC). The aim of this study was to relate the HPC count to CD34(+) cell levels in mobilized peripheral blood and to determine whether the HPC count was valuable in predicting apheresis yields of CD34(+) cells. Studies were performed on 114 samples from 67 patients undergoing progenitor cell mobilization.HPC cells were undetectable in the steady state. On the day of apheresis the HPC and CD34 counts were weakly correlated, with the median KPC count being 2.3-fold greater than the CD34(+) cell count. The HPC count did not include the CD34(+) cells as immunomagnetic depletion of CD34(+) cells did not significantly reduce the HPC count. CD34(+) cell counts predicted for apheresis yield (r=0.773) on that day as did the HPC count (r=0.623). The optimal strategy to prevent unnecessary harvesting while minimizing the risk of missing an adequate harvest, and minimizing laboratory investigations, was to screen all samples for HPC and limit CD34(+) cell measurements to those with an HPC count