Functions and imaging of mast cell and neural axis of the gut.
Functions and imaging of mast cell and neural axis of the gut.
复制标题
DOI：
10.1053/j.gastro.2013.01.040
复制
发表时间：
2013-04
期刊：
影响因子：
29.4
通讯作者：
Camilleri M
中科院分区：
文献类型：
--
作者：
Schemann M;Camilleri M
肠壁中神经和肥大细胞之间的密切联系为这些元素之间的功能相互作用提供了微观解剖学基础，支持肥大细胞-神经轴影响健康和疾病中的肠道功能的假设。先进的形态学和成像技术，现在可用于评估在人类肠道组织中的肥大细胞神经轴的结构和功能的关系。形态学技术，包括共同标记肥大细胞和神经，以评估其密度和解剖接近的变化。钙（Ca++）和电位染料成像提供了新的见解功能，如肥大细胞神经信号在人类肠道组织。这种成像有望揭示新的离子或分子靶点，以使由肥大细胞过度活跃诱导的神经致敏或由神经源性炎症途径诱导的肥大细胞致敏正常化。这些靶点包括蛋白酶激活受体（PAR）1或组胺受体。在患者中，体内肠道中的光学成像具有识别体内神经结构和炎症的潜力。后者有一定的风险和潜在的采样错误与单一活检。不需要造影剂的视网膜神经纤维成像技术（光学相干断层扫描和全视野光学相干显微镜）可应用于研究粘膜下神经丛。此外，粘膜下剥离，使用荧光标记物，和内窥镜共聚焦显微镜的组合提供了肌间神经元和平滑肌细胞在固有肌层的详细成像。胃肠动力和功能性疾病的研究是可行的，而不需要全层活检。
Close association between nerves and mast cells in the gut wall provides the microanatomic basis for functional interactions between these elements, supporting the hypothesis that a mast cell–nerve axis influences gut functions in health and disease. Advanced morphology and imaging techniques are now available to assess structural and functional relationships of the mast cell–nerve axis in human gut tissues. Morphologic techniques including co-labeling of mast cells and nerves serve to evaluate changes in their densities and anatomic proximity. Calcium (Ca++) and potentiometric dye imaging provide novel insights into functions such as mast cell–nerve signaling in the human gut tissues. Such imaging promises to reveal new ionic or molecular targets to normalize nerve sensitization induced by mast cell hyperactivity or mast cell sensitization by neurogenic inflammatory pathways. These targets include proteinase-activated receptor (PAR) 1 or histamine receptors. In patients, optical imaging in the gut in vivo has the potential to identify neural structures and inflammation in vivo. The latter has some risks and potential of sampling error with a single biopsy. Techniques that image nerve fibers in the retina without the need for contrast agents (optical coherence tomography and full-field optical coherence microscopy) may be applied to study submucous neural plexus. Moreover, the combination of submucosal dissection, use of a fluorescent marker, and endoscopic confocal microscopy provides detailed imaging of myenteric neurons and smooth muscle cells in the muscularis propria. Studies of motility and functional gastrointestinal disorders would be feasible without the need for full-thickness biopsy.
登录
查看更多内容
影响因子：
29.4
作者：
Buhner, Sabine;Li, Qin;Schemann, Michael
通讯作者：
Schemann, Michael
影响因子：
5.5
作者：
Breunig, Eva;Michel, Klaus;Schemann, Michael
通讯作者：
Schemann, Michael
影响因子：
29.4
作者：
Barbara, Giovanni;Wang, Bingxian;Corinaldesi, Roberto
通讯作者：
Corinaldesi, Roberto
DOI：
10.1152/ajpgi.00528.2003
发表时间：
2004-07-01
影响因子：
4.5
作者：
De Jonge, F;De Laet, A;Kroese, ABA
通讯作者：
Kroese, ABA
影响因子：
3.6
作者：
Beaurepaire, E;Boccara, AC;Saint-Jalmes, H
通讯作者：
Saint-Jalmes, H