Gold nanoparticles: dispersibility in biological media and cell-biological effect
Gold nanoparticles: dispersibility in biological media and cell-biological effect
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DOI：
10.1039/c0jm01071e
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Epple, Matthias
中科院分区：
文献类型：
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作者：
Mahl, Dirk;Greulich, Christina;Epple, Matthias
制备了流体动力学直径在25和37 nm之间的球形金纳米颗粒，并用聚（N-乙烯基吡咯烷酮）（PVP）或三（间磺酸钠苯基）膦（TPPTS）稳定。将它们置于不同的细胞培养基中，G.纯RPMI、含有高达10%胎牛血清（FCS）的RPMI和含有高达10%牛血清白蛋白（BSA）的RPMI，并通过动态光散射研究团聚率。在纯RPMI中，观察到强烈的附聚，而在RPMI-FCS和RPMI-BSA混合物中，颗粒在高于1重量%蛋白质浓度时保持良好分散。还研究了PVP稳定的金纳米颗粒对人间充质干细胞（hMSC）的影响。在用金纳米颗粒孵育hMSC后，未观察到对存活力和趋化性的显著影响。然而，金纳米颗粒诱导hMSC的活化，如IL-6和IL-8的释放所示。
Spherical gold nanoparticles with a hydrodynamic diameter between 25 and 37 nm were prepared and stabilised with poly(N-vinylpyrrolidone) (PVP) or tris(sodium-m-sulfonatophenyl) phosphine (TPPTS). They were subjected to different cell culture media, e. g. pure RPMI, RPMI containing up to 10% of fetal calf serum (FCS), and RPMI containing up to 10% of bovine serum albumin (BSA), and the rate of agglomeration was studied by dynamic light scattering. In pure RPMI, a strong agglomeration was observed whereas in the RPMI-FCS and RPMI-BSA mixtures the particles remained well dispersed above 1 wt% protein concentration. The effect of PVP-stabilised gold nanoparticles on human mesenchymal stem cells (hMSC) was studied as well. No significant influence on the viability and chemotaxis was observed after incubation of hMSC with gold nanoparticles. However, gold nanoparticles induced the activation of hMSC as indicated by the release of IL-6 and IL-8.