Expression of Xhdsi-1VOC, a novel member of the vicinal oxygen chelate (VOC) metalloenzyme superfamily, is up-regulated in leaves and roots during desiccation in the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.
Expression of Xhdsi-1VOC, a novel member of the vicinal oxygen chelate (VOC) metalloenzyme superfamily, is up-regulated in leaves and roots during desiccation in the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.
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DOI：
10.1093/jxb/ern226
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发表时间：
2008
影响因子：
6.9
通讯作者：
Illing N
中科院分区：
文献类型：
--
作者：
Mulako I;Farrant JM;Collett H;Illing N
新植物基因的注释通常基于与已知蛋白质基序的序列和结构相似性。然而，了解这些基因的生物学功能取决于确定它们被激活的条件。复活植物矮生旱生植物是鉴定和表征脱水耐性基因的良好模式系统。干燥诱导基因1（dsi-1VOC）是一个以前未被鉴定的植物基因，在X.卑微的副艇长humilis dehydration induced-1基因Xhdsi-1VOC与邻位氧螯合物（VOC）金属酶超家族具有结构同源性。这个超家族中的蛋白质几乎没有序列相似性，但其特征在于共同的βα β结构折叠。已经鉴定了许多XhDsi-1VOC的植物直向同源物，包括拟南芥At 1g 07645，其目前被注释为glycoprotein酶I样基因，以及许多来自种子cDNA文库的EST。然而，Xhdsi-1VOC及其直向同源物不包含谷胱甘肽和锌结合位点，这些位点在谷胱甘肽酶I基因中是保守的。此外，Xhdsi-1VOC在酵母中的表达未能拯救酵母谷胱甘肽酶I突变体。At 1g 07645的信使RNA转录物在种子成熟过程中积累，但在拟南芥营养组织中不受失水、盐或甘露醇胁迫的诱导。因此，dsi-1VOC基因是干旱敏感植物中的一个种子特异性基因，在复苏植物X中，该基因被营养组织中的失水激活。humilis，并在允许植物组织在失去95%的相对含水量后存活方面发挥重要作用。
The annotation of novel plant genes is frequently based on sequence and structural similarity to known protein motifs. Understanding the biological function of these genes is dependent on identifying conditions under which they are activated, however. The resurrection plant, Xerophyta humilis is a good model system for identifying and characterizing genes which are important for desiccation tolerance. Desiccation induced-1 (dsi-1VOC), a previously uncharacterized plant gene, is up-regulated during desiccation in leaves, roots, and seeds in X. humilis. The X. humilis desiccation induced-1 gene, Xhdsi-1VOC, shares structural homology with the vicinal oxygen chelate (VOC) metalloenzyme superfamily. Proteins in this superfamily share little sequence similarity, but are characterized by a common βαβββ structural fold. A number of plant orthologues of XhDsi-1VOC have been identified, including Arabidopsis thaliana At1g07645, which is currently annotated as a glyoxalase I-like gene, and many ESTs derived from seed cDNA libraries. Xhdsi-1VOC and its orthologues do not, however, contain the glutathione and zinc binding sites conserved in glyoxalase I genes. Furthermore, expression of Xhdsi-1VOC in yeast failed to rescue a yeast glyoxalase I mutant. Messenger RNA transcripts for At1g07645 accumulate during seed maturation, but are not induced by water loss, salt or mannitol stress in vegetative tissue in Arabidopsis. It is concluded that dsi-1VOC is a seed-specific gene in desiccation-sensitive plants that is activated by water loss in vegetative tissues in the resurrection plant X. humilis and plays an important role in allowing plant tissues to survive loss of 95% of their relative water content.
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影响因子：
2.7
作者：
GAFF, DF
通讯作者：
GAFF, DF
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