Myosin isoforms in neonatal rat extensor digitorum longus, diaphragm, and soleus muscles.
Myosin isoforms in neonatal rat extensor digitorum longus, diaphragm, and soleus muscles.
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新生大鼠趾长伸肌、膈肌和比目鱼肌中的肌球蛋白亚型。
DOI：
10.1152/ajplung.1990.259.2.l116
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ontell,M
中科院分区：
文献类型：
--
作者：
LaFramboise,WA;Daood,MJ;Guthrie,RD;Butler-Browne,GS;Whalen,RG;Ontell,M
采用电泳和免疫组化技术比较了大鼠趾长伸肌、膈肌和比目鱼肌中胚胎和新生儿肌球蛋白亚型的出生后消除。天然肌球蛋白的电泳显示，新生儿带存在于所有三个肌肉在第4天，但没有从第21天伸趾长肌，表现出其成人电泳模式。成熟的电泳带型分别出现在第60天和第125天的膈肌和比目鱼肌。免疫组化分析表明，胚胎肌球蛋白重链是存在于所有的第4天的样品，但缺席的第21天。定量评价确定，新生儿肌球蛋白重链（MHCneo）的消除速度在趾长伸肌比在膈肌更快，比目鱼肌具有最慢的消除速度的这种亚型。胚胎肌球蛋白轻链通过双向电泳检测到8天在每个肌肉。这些数据表明，MHCneo的出生后消除是组织特异性和时间依赖性的，但不受活动水平或头尾位置的影响。
The postnatal elimination of embryonic and neonatal myosin isoforms in the rat extensor digitorum longus, diaphragm, and soleus muscles was compared using electrophoresis and immunohistochemical techniques. Electrophoresis of native myosin showed that neonatal bands were present in all three muscles on day 4 but were absent from the day 21 extensor digitorum longus muscle that exhibited its adult electrophoretic pattern. Mature electrophoretic banding patterns were present on days 60 and 125 in the diaphragm and soleus muscles, respectively. Immunohistochemical analysis indicated that embryonic myosin heavy chain was present in all day 4 samples but absent by day 21. Quantitative evaluation determined that the rate of elimination of neonatal myosin heavy chain (MHCneo) was faster in the extensor digitorum longus muscle than in the diaphragm, with the soleus muscle having the slowest rate of elimination of this isoform. Embryonic myosin light chain was detected by two-dimensional electrophoresis through day 8 in each of the muscles. These data indicate that postnatal elimination of MHCneo is tissue specific and time dependent but not governed by either activity level or rostral-caudal position.