High expression of microRNA-183/182/96 cluster as a prognostic biomarker for breast cancer.
High expression of microRNA-183/182/96 cluster as a prognostic biomarker for breast cancer.
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microRNA-183/182/96簇的高表达作为乳腺癌的预后生物标志物
DOI：
10.1038/srep24502
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发表时间：
2016-04-13
影响因子：
4.6
通讯作者：
Xie X
中科院分区：
文献类型：
--
作者：
Song C;Zhang L;Wang J;Huang Z;Li X;Wu M;Li S;Tang H;Xie X
因此，迫切需要更敏感、更有效的乳腺癌诊断标志物。microRNA-183/182/96簇已被报道参与多种癌症的肿瘤发生和进展，并且它是一种有前途的癌症预后生物标志物。本研究的目的是确定miR-183/182/96簇在乳腺癌组织中的表达水平，并评估其在乳腺癌中的预后作用。采用实时定量聚合酶链反应（qRT-PCR）检测41例乳腺癌组织、癌旁正常组织（对照组织）及不同乳腺细胞系中miR-183/182/96簇的表达水平。使用131个组织微阵列（TMA）上miR-183/182/96簇的原位杂交（ISH）来统计分析其预后作用。乳腺癌组织中miR-183/182/96簇水平显著高于对照组织。miR-183/182/96簇在人乳腺癌细胞系中也上调。增加的miR-183/182/96簇水平与局部复发、远处转移和不良临床结局相关。我们的发现提高了我们对miR-183/182/96簇在乳腺癌中表达水平的理解，并阐明了miR-183/182/96簇作为乳腺癌新的预后生物标志物的作用。
More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers, and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.