Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells.
Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells.
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在离体龟结肠上皮细胞中利多卡因和奎尼丁阻断单一钾通道。
DOI：
10.1152/ajpcell.1986.251.1.c85
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dawson,DC
中科院分区：
文献类型：
--
作者：
Richards,NW;Dawson,DC
用膜片钳技术记录透明质酸酶分离的单个甲鱼结肠上皮细胞膜上单通道电流。使用康宁#7052玻璃制成的电极，这些电池始终形成高阻密封。在低K值(2.5 mm)和高K值(114.5 mm)的细胞上记录到外向钾电流，其斜率电导为17ps，反转电位大于-70 mV。通过K通道的电流被利多卡因、奎尼丁和钡所阻断。这些药物还阻断了通过宏观电流测量在完整的结肠上皮基侧膜上发现的细胞肿胀诱导的K电导，这表明通过单通道记录在分离的甲鱼结肠细胞中识别的17pS K通道可能是这种宏观定义的K电导的原因。
The patch-clamp technique for recording single-channel currents across cell membranes was applied to single turtle colon epithelial cells isolated with hyaluronidase. With electrodes fabricated from Corning #7052 glass, high-resistance seals were consistently formed to these cells. In on-cell patches with low K (2.5 mM) in the pipette and high K (114.5 mM) in the bath, outward K currents were recorded that had a slope conductance of 17 pS and a reversal potential greater than -70 mV. Currents through this K channel were blocked by lidocaine, quinidine, and barium. These agents also block a cell swelling-induced K conductance identified by macroscopic current measurements in the basolateral membranes of the intact colonic epithelium, suggesting that the 17 pS K channel identified by single-channel recording in isolated turtle colon cells may be responsible for this macroscopically defined K conductance.
兔皮质集合管顶膜钙激活钾通道的单通道记录。
DOI：
10.1073/pnas.81.13.4237
发表时间：
1984
影响因子：
11.1
作者：
Hunter,M;Lopes,AG;Boulpaep,EL;Giebisch,GH
通讯作者：
Giebisch,GH
局部麻醉剂暂时阻断通过单个乙酰胆碱受体通道的电流。
DOI：
--
发表时间：
1978
期刊：
Journal of Physiology
影响因子：
--
作者：
E. Neher;J. Steinbach
通讯作者：
J. Steinbach