Genome-Wide Identification of R2R3-MYB Genes and Expression Analyses During Abiotic Stress in Gossypium raimondii.
Genome-Wide Identification of R2R3-MYB Genes and Expression Analyses During Abiotic Stress in Gossypium raimondii.
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DOI：
10.1038/srep22980
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发表时间：
2016-03-24
影响因子：
4.6
通讯作者：
Zhang B
中科院分区：
文献类型：
--
作者：
He Q;Jones DC;Li W;Xie F;Ma J;Sun R;Wang Q;Zhu S;Zhang B
R2R3-MYB是最大的转录因子家族之一，与多种生物过程有关。不同植物中R2R3-MYB基因的数量差异很大。然而，该基因家族在棉花中的全基因组鉴定尚未见报道。本研究共在棉花D基因组（Gossypium raimondii）中鉴定出205个推测的R2R3-MYB基因，这些基因比在其他经济作物中发现的基因组大得多。从拟南芥和水稻中提取的R2R3-MYB基因将这些grmyb分为13组。对氨基酸基序和系统发育树进行了预测和分析。grmyb序列以不同的密度分布在13条染色体上。结果表明，雷蒙地鼠R2R3-MYB家族的扩增主要是由于全基因组重复和片段重复。在不同的非生物胁迫条件下，检测了52个GrMYBs和46个GaMYBs在根和叶中的表达模式。结果表明，棉花MYB基因在盐胁迫和干旱胁迫下存在差异表达。我们的研究结果将有助于确定MYB基因在作物改良过程中对胁迫反应的确切作用。
The R2R3-MYB is one of the largest families of transcription factors, which have been implicated in multiple biological processes. There is great diversity in the number of R2R3-MYB genes in different plants. However, there is no report on genome-wide characterization of this gene family in cotton. In the present study, a total of 205 putative R2R3-MYB genes were identified in cotton D genome (Gossypium raimondii), that are much larger than that found in other cash crops with fully sequenced genomes. These GrMYBs were classified into 13 groups with the R2R3-MYB genes from Arabidopsis and rice. The amino acid motifs and phylogenetic tree were predicted and analyzed. The sequences of GrMYBs were distributed across 13 chromosomes at various densities. The results showed that the expansion of the G. Raimondii R2R3-MYB family was mainly attributable to whole genome duplication and segmental duplication. Moreover, the expression pattern of 52 selected GrMYBs and 46 GaMYBs were tested in roots and leaves under different abiotic stress conditions. The results revealed that the MYB genes in cotton were differentially expressed under salt and drought stress treatment. Our results will be useful for determining the precise role of the MYB genes during stress responses with crop improvement.