Neonatal murine macrophages show enhanced chemotactic capacity upon toll-like receptor stimulation
Neonatal murine macrophages show enhanced chemotactic capacity upon toll-like receptor stimulation
复制标题
新生鼠巨噬细胞在 Toll 样受体刺激后表现出增强的趋化能力
DOI：
10.1007/s00383-013-3457-7
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发表时间：
2014
影响因子：
1.8
通讯作者：
Kuebler JF
中科院分区：
文献类型：
--
作者：
Winterberg T;Vieten G;Feldmann L;Hansen G;Hennig C;Ure BM;Kuebler JF
研究背景新生儿外科手术患者常受到大量炎症反应的威胁。新生儿巨噬细胞是这一过程中的关键参与者。我们研究了新生儿巨噬细胞在暴露于不同的细菌或病毒配体Toll样受体（TLRs）后启动局部炎症反应的能力。方法通过腹腔灌洗获得新生儿（<24 h）和成年（42天）C57 BL/6 J小鼠的腹膜冲洗液。在第一组实验中，纯化巨噬细胞并通过四种不同的TLR配体刺激6小时。提取mRNA用于转录组分析。在第二组实验中，将脂多糖施加到腹膜腔中。孵育6小时后，发炎腔的细胞组成进行了评估，通过细胞学染色以及chipcytometry.ResultsNeonatal小鼠腹腔巨噬细胞的亲和抗趋化基因的表达显着不同。这些基因的功能分配显示增强的新生儿巨噬细胞的趋化潜力，并证实了更高的促炎细胞流入新生儿腹膜cavities.ConclusionNeonatal腹膜巨噬细胞表现出增强的趋化潜力后，刺激与TLR配体。这与炎性细胞流入腹膜腔的增加有关。这可能有助于新生儿和早产儿的强烈炎症反应。
BackgroundThe neonatal surgical patient is threatened by exuberant inflammatory reactions. Neonatal macrophages are key players in this process. We investigated the ability of neonatal macrophages to initiate a local inflammatory reaction upon exposure to different bacterial or viral ligands to toll-like receptors (TLRs).MethodsPeritoneal wash outs from neonatal (<24 h) and adult (42 days) C57BL/6J mice were gained by peritoneal lavages. In a first set of experiments, macrophages were purified and stimulated for 6 h by four different TLR ligands. mRNA was extracted for transcriptome analysis. In a second set of experiments, lipopolysaccharide was applied into peritoneal cavities. After 6 h of incubation, the cellular composition of the inflamed cavities was evaluated by cytological staining as well as chipcytometry.ResultsNeonatal murine peritoneal macrophages differed significantly in the expression of pro- and anti-chemotactic genes. Functional assignment of these genes revealed enhanced chemotactic potential of neonatal macrophages and was confirmed by a higher influx of pro-inflammatory cells into neonatal peritoneal cavities.ConclusionNeonatal peritoneal macrophages demonstrated an enhanced chemotactic potential upon stimulation with four TLR ligands. This was associated with an increased influx of inflammatory cells to the peritoneal cavity. This might contribute to the strong inflammatory responses of neonates and preterms.