Extracellular accumulation of mono- and di-succinoyl trehalose lipids by a strain of Rhodococcus erythropolis grown on n-alkanes.
Extracellular accumulation of mono- and di-succinoyl trehalose lipids by a strain of Rhodococcus erythropolis grown on n-alkanes.
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在正烷烃上生长的红平红球菌菌株对单琥珀酰海藻糖脂质和二琥珀酰海藻糖脂质的细胞外积累。
DOI：
10.1271/bbb1961.53.757
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
T. Tabuchi
中科院分区：
文献类型：
--
作者：
Yasukazu Uchida;R. Tsuchiya;M. Chino;J. Hirano;T. Tabuchi
在碱性条件下从土壤中分离出的细菌菌株 Sd-74，被发现能够从正烷烃中大量产生酸性胞外脂。该菌株被证明是耐碱而非嗜碱的，被鉴定为红平红球菌。从含有正十六烷作为唯一碳源的培养液（11）中分离出酸性胞外脂质（15 g），并发现其由两种新的琥珀酰海藻糖脂质（STL-1和STL-2）组成。纯化后，STL-1 的熔点为 169 至 171°C（c = 0.6，CHCl3/MeOH = 2:1），STL-2 的熔点为 161 至 163°C（c = 0.6，CHCl3/MeOH = 2:1）。根据化学降解和甲基化结果，STL-1和STL-2分别为2,3,4,2′-di-6>-琥珀酰基-二-O-烷酰基-a,a-海藻糖和2,3,4-单-O-琥珀酰基-二-O-烷酰基-a,a-海藻糖。
A bacterium, strain Sd-74, which was isolated from soil under alkaline conditions, was found to abundantly produce acidic exolipids from n-alkanes. The strain proved to be alkali-resistant rather than alkalophilic and was identified as Rhodococcus erythropolis.The acidic exolipids (15 g) were isolated from culture broth (11) containing n-hexadecane as the sole carbon source and were found to be composed of two new succinoyl trehalose lipids (STL-1 and STL-2). After purification, STL-1 showed mp 169 to 171°C and (c = 0.6, CHC13/MeOH = 2:1), and STL-2 mp 161 to 163°C and (c = 0.6, CHCl3/MeOH = 2:1). On the basis of the results of chemical degradation and methylation, STL-1 and STL-2 were concluded to be 2,3,4,2′-di-6>-succinoyl-di-O-alkanoyl-a,a-trehalose and 2,3,4-mono-O-succinoyl-di-O-alkanoyl-a,a-trehalose, respectively.