Variations in abundance and community composition of denitrifying bacteria during a cyanobacterial bloom in a eutrophic shallow lake in China
Variations in abundance and community composition of denitrifying bacteria during a cyanobacterial bloom in a eutrophic shallow lake in China
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中国富营养化浅湖蓝藻水华期间反硝化细菌丰度和群落组成的变化
DOI：
10.1080/02705060.2017.1323681
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发表时间：
2017
影响因子：
1.3
通讯作者：
Yan SH
中科院分区：
文献类型：
--
作者：
Zhang Weiguo;Gao Yan;Yi Neng;Wang Chengcheng;Di Panpan;Yan Shaohua;Yan SH
虽然蓝藻水华可以改变微生物群落，但目前还不清楚这种有害的水华会对分解细菌产生什么影响，而分解细菌是去除水中过量氮的驱动因素。为了阐明这一影响，利用实时定量聚合酶链反应（qPCR）和454焦磷酸测序技术，对中国东南部富营养化浅水湖泊中蓝藻周期性增殖的蓝藻种群进行了研究。当蓝藻密度逐渐增加时，在三个月的时间内定期收集样品。在qPCR分析中，nirK、nirSandnosZ的丰度与蓝藻生物量呈强正相关。此外，454-焦磷酸测序显示，随着蓝藻密度的增加，浮游细菌的群落组成发生了变化。这些结果表明，蓝藻水华的发生可能与反硝化细菌群落组成的变化有关，从而可能导致富营养化水体中反硝化作用的改变。
Although cyanobacterial blooms can change microbial communities, it is still unclear what impact such harmful blooms will have on denitrifying bacteria, the drivers of the removal of excessive nitrogen from water. In order to clarify the impact, populations of denitrifying bacteria, with periodic proliferation and dominance of cyanobacteria in a eutrophic shallow lake located in southeast China, were investigated using quantitative real-time polymerase chain reaction (qPCR) and 454-pyrosequencing based on the copper-containing nitrite reductase (nirK) gene, cytochrome cd1-containing nitrite reductase (nirS) gene and nitrous oxide reductase (nosZ) gene. Samples were collected periodically during a three-month period when the cyanobacterial density gradually increased. In the qPCR analyses, abundances ofnirK,nirSandnosZwere intensely positively correlated with the biomass of cyanobacteria. Moreover, 454-pyrosequencing revealed that the community composition of denitrifying bacteria shifted with the increase in cyanobacterial density. These results indicated that the shifts of the community composition of denitrifying bacteria might be related to cyanobacterial blooms, which could potentially lead to alterations of denitrification in eutrophic water.