Joints formed by RecA protein from oligonucleotides and duplex DNA block initiation and elongation of transcription.
Joints formed by RecA protein from oligonucleotides and duplex DNA block initiation and elongation of transcription.
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由寡核苷酸和双链 DNA 组成的 RecA 蛋白形成的接头可阻断转录的起始和延伸。
DOI：
10.1093/nar/20.12.3121
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发表时间：
1992
影响因子：
14.9
通讯作者：
Radding,CM
中科院分区：
文献类型：
--
作者：
Golub,EI;Ward,DC;Radding,CM
在ATP的非水解类似物ATPγS存在下，RecA蛋白可粘附在寡脱氧核苷酸上，形成稳定的可在双链DNA中找到其同源序列的寡脱氧核苷酸丝。寡核苷酸和双链体DNA形成的同源连接只有在RecA蛋白不被去除时才是稳定的。这种核蛋白接头覆盖T3或T7噬菌体RNA聚合酶的部分或全部启动子区，阻断由这些聚合酶指导的转录。位于RNA聚合酶启动子下游的相同类型的接头也抑制转录的延伸并引起截短的转录物的积累。这些观察结果表明，RecA蛋白可用于关闭任何已知序列的启动子的转录。
In the presence of the non-hydrolyzable analog of ATP, ATPγS, RecA protein can polymerize on an oligodeoxyribonucleotide to form a stable oligonucleoprotein filament that can find its homologous sequence in double-stranded DNA. The homologous joint formed by the oligonucleotide and duplex DNA is stable only if RecA protein is not removed. Such a nucleoprotein joint, covering a part or all of the promoter region of T3 or T7 phage RNA polymerase, blocked transcription directed by those polymerases. The same kind of joint, located downstream of the RNA polymerase promoter, also inhibited elongation of transcription and caused accumulation of truncated transcripts. These observations suggest that RecA protein can be used to shut off transcription from any promoter of known sequence.