Complex Molecular Evolution and Expression of Expansin Gene Families in Three Basic Diploid Species of Brassica
Complex Molecular Evolution and Expression of Expansin Gene Families in Three Basic Diploid Species of Brassica
复制标题
三种基本二倍体芸苔属植物扩展蛋白基因家族的复杂分子进化和表达
DOI：
10.3390/ijms21103424
复制
发表时间：
2020-05-01
影响因子：
5.6
通讯作者：
Cao, Jiashu
中科院分区：
文献类型：
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作者：
Liu, Weimiao;Lyu, Tianqi;Cao, Jiashu
Expansins是植物细胞壁的一种结构蛋白，它们通过松弛细胞壁来扩大细胞。因此，Expansins参与许多生长和发育过程。甘蓝型油菜、甘蓝型油菜和黑甘蓝的全基因组序列为研究扩展蛋白基因提供了有效的平台，可以与拟南芥的类似物进行比较。本研究鉴定并鉴定了油菜、甘蓝和黑菜中的Expansin家族。通过系统发育、基因结构和理化性质的比较分析，将Expansin家族划分为4个亚家族，并据此探讨了它们的扩展模式和进化细节。结果表明，这3个物种在从拟南星天牛中分离出来后，经过独立进化后，3个物种中的Expansin家族相似性增加，但差异较小。通过搜索启动子和编码序列的差异，发现拟南芥与三个基本物种的同源基因之间存在显著的正相关。随后，差异表达表明这三个物种的Expansin家族存在广泛的功能差异，特别是在生殖发育方面。因此，这些结果支持了油菜属基本种的分子进化、这些基因的潜在功能以及相关作物的遗传改良。
Expansins are a kind of structural proteins of the plant cell wall, and they enlarge cells by loosening the cell walls. Therefore, expansins are involved in many growth and development processes. The complete genomic sequences of Brassica rapa, Brassica oleracea and Brassica nigra provide effective platforms for researchers to study expansin genes, and can be compared with analogues in Arabidopsis thaliana. This study identified and characterized expansin families in B. rapa, B. oleracea, and B. nigra. Through the comparative analysis of phylogeny, gene structure, and physicochemical properties, the expansin families were divided into four subfamilies, and then their expansion patterns and evolution details were explored accordingly. Results showed that after the three species underwent independent evolution following their separation from A. thaliana, the expansin families in the three species had increased similarities but fewer divergences. By searching divergences of promoters and coding sequences, significant positive correlations were revealed among orthologs in A. thaliana and the three basic species. Subsequently, differential expressions indicated extensive functional divergences in the expansin families of the three species, especially in reproductive development. Hence, these results support the molecular evolution of basic Brassica species, potential functions of these genes, and genetic improvement of related crops.