INFECTIVITY OF CULTURED PLASMODIUM-FALCIPARUM GAMETOCYTES TO MOSQUITOS
INFECTIVITY OF CULTURED PLASMODIUM-FALCIPARUM GAMETOCYTES TO MOSQUITOS
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DOI：
10.1017/s0031182000062065
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发表时间：
1989-04-01
期刊：
影响因子：
2.4
通讯作者：
MEUWISSEN, JHET
中科院分区：
文献类型：
--
作者：
PONNUDURAI, T;LENSEN, AHW;MEUWISSEN, JHET
本文考虑了可能影响常规和高水平的蚊子感染恶性疟原虫配子体的各种因素。其中最重要的是选择合适的分离物，具有冷冻保存设施和良好的培养起始技术。使用自动化培养系统，严格遵守细节和常规，消除了大部分的变异性。用于配子母细胞培养和包含在蚊子饲料材料中的血清的质量是最重要的。用于培养目的的血液收集必须优选地包括酒精作为用于清洁供体皮肤或合适的容器的防腐剂。Moscow血餐不应包括含柠檬酸盐磷酸盐葡萄糖的血浆或收集在微量采血管中的血清或来自接受普鲁胍-氯喹预防的志愿者的血清。单独服用氯喹的个体血清不影响传播。5 - 10%的血细胞比容允许培养同样感染性的配子母细胞。根据雌性配子母细胞或配子的数量来预测蚊子感染的结果是不可能的。在任何实验中，卵囊负荷最初增加，随后下降，配子母细胞的数量逐渐减少，伴随着传播效率的逐渐增加。实验中蚊子感染的一些变异性是由于蚊子血液消化速度的个体差异。描述了一种新的膜进料器，其具有三种不同的尺寸以适应各种目标。新的设计允许更多的蚊子在有限的时间内喂养相比，旧的更广泛使用的喂食器相同的容量，和蚊子喂养的新型喂食器往往比那些喂养的旧类型更好地感染。
Various factors that may influence routine and high levels of mosquito infection with cultured Plasmodium falciparum gametocytes are considered in this paper. One of the most important is the choice of an appropriate isolate, with facilities for cryopreservation and a good technique for initation of cultures. The use of automated culture systems with strict adherence to detail and routine has eliminated much of the variability. The quality of the serum used for the culture of gametocytes and inclusion in the feed material for mosquitoes is of the highest importance. Blood collection for culture purposes must preferably involve alcohol as an antiseptic for cleaning donor skin or suitable receptacles. Mosquito blood meals should not include plasma with citrate phosphate dextrose or sera collected in microtainer tubes or from volunteers on proguanil-chloroquine prophylaxis. Sera of individuals on chloroquine alone do not influence transmission. Haematocrits of from 5 to 10% permit the culture of equally infective gametocytes. It was immpossible to predict the outcome of an infection in mosquitoes based on the number of female gametocytes or gametes. Within any experiment, the oocyst load initially increased, followed by a decline with progressively lower numbers of gametocytes accompanied by a progressive increase in the efficiency of transmission. Some of the variability of mosquito infection within an experiment was due to individual differences in the speed of blood digestion of the mosquitoes. A new membrane feeder is described with three different sizes to accommodate a variety of goals. The new design permitted more mosquitoes to feed within a limited period compared to the older more widely used feeder of equivalent capacity, and mosquitoes feeding on the new-type feeder were frequently better infected than those fed on the older type.