Bacterial flora of the human oral cavity, and the upper and lower esophagus
Bacterial flora of the human oral cavity, and the upper and lower esophagus
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DOI：
10.1111/j.1442-2050.2012.01328.x
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Bove, M.
中科院分区：
文献类型：
--
作者：
Grusell, E. Norder;Dahlen, G.;Bove, M.
本参考研究旨在调查健康人类下段食管的细菌菌群，并将其与上食管和口腔粘膜的细菌菌群进行比较。除了刷样本之外，使用活检不仅可以包含表面上存在的瞬时细菌，还可以包含驻留在上皮细胞中的细菌，并且可以比较两种方法的产量。包括 40 名因对食管菌群无已知影响且没有食管疾病症状或内镜征象而计划进行手术的患者。使用密封刷和活检钳从口腔、上食管和下食管粘膜采集样本。对琼脂平板上培养的菌落进行分类和半定量。鉴定出二十三种不同的细菌种类，三个地点都存在相似的菌株。最常见的细菌是草绿色链球菌，在刷子样本和活检组织中的出现率分别为 98% 和 95%。发生在口腔、上食管和下食管的物种中位数在 3 到 4 之间（范围 07）。与食道中的任一水平相比，口腔中的物种总数显着更高，而刷检和活检取样获得的产量高度相关。因此，正常人的食道有其自身的常驻菌群，这与口腔粘膜的菌群相似。该植物群由多种物种组成，但数量相对较少。食道中最常见的寄生菌是链球菌，在刷子样本和活组织检查中的出现率为 9598%。有必要对嗜酸粒细胞性食管炎和胃食管反流病患者进行比较研究。
This reference study aims to survey the bacterial flora of the healthy lower human esophagus and to compare it with that of the upper esophagus and oral mucosa. The use of biopsies, in addition to brush samples, allows inclusion of not only transient bacteria present on the surface but also bacteria residing in the epithelia, and the yield of the two methods can be compared. Forty patients scheduled for surgery for reasons with no known influence on esophageal flora and with no symptoms or endoscopic signs of esophageal disease were included. Samples were collected from the oral, upper esophageal, and lower esophageal mucosa using sealed brushes and biopsy forceps. Colonies cultivated on agar plates were classified and semiquantified. Twenty-three different bacterial species were identified, with similar strains present at the three sites. The most common group of bacteria was viridans streptococci, with an occurrence rate in brush samples and biopsies of 98% and 95%, respectively. The median number of species occurring in the oral cavity, upper esophagus, and lower esophagus was between 3 and 4 (range 07). The total number of species in the oral cavity was significantly higher when compared with either level in the esophagus, while the yields obtained by brush and biopsy sampling were highly correlated. Hence, the normal human esophagus is colonized with a resident bacterial flora of its own, which has similarities to that of the oral mucosa. There are diverse species that make up this flora, although in relatively low amounts. The most frequent inhabitants of the esophagus are streptococci, with an occurrence rate in brush samples and biopsies of 9598%. Comparative studies of patients with eosinophilic esophagitis and gastroesophageal reflux disease are warranted.