Variation in antigen-antibody af!nity among serotypes of Salmonella O4 serogroup, determined using speci!c antisera
Variation in antigen-antibody af!nity among serotypes of Salmonella O4 serogroup, determined using speci!c antisera
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使用特异性抗血清测定沙门氏菌 O4 血清群血清型之间抗原抗体亲和力的变化
DOI：
10.1093/femsle/fnv168
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发表时间：
2015
影响因子：
2.1
通讯作者：
Yoshihiro Shimoji and Masahiro Eguchi
中科院分区：
文献类型：
--
作者：
Swarmistha Devi Aribam;Marta Elsheimer-Matulova;Hidenori Matsui;Jiro Hirota1;Kazumasa Shiraiwa;Yohsuke Ogawa;Hirokazu Hikono;Yoshihiro Shimoji and Masahiro Eguchi
血清分型广泛用于沙门氏菌的分型，依赖于内毒素O抗原和鞭毛蛋白(H抗原)成分的测定。由于O-抗原是高度可变的，在结构上对每个血清型都是独一无二的，我们通过免疫印迹和酶联免疫吸附试验研究了O4血清群沙门氏菌亚型的内毒素与特异性抗抗原血清的结合亲和力。由于O4血清群的血清型也表达O-抗原因子12，因此也使用了O12抗血清进行分析。不同血清型的内毒素与抗血清的结合亲和力不同。因此，在抗原抗体亲和力的基础上，用O4和O12抗血清进行了改良的凝集试验。虽然O4血清群的血清型具有共同的O-抗原因子4和12，但分析表明，每个血清型的凝集反应程度不同。我们认为，尽管存在共同的O-抗原因子4和12，但O4群沙门氏菌血清型与特定抗血清表现出不同的结合亲和力。
Serotyping is widely used for typingSalmonelladuring surveillance, and depends on determining the lipopolysaccharide (LPS) O-antigen and the flagellar protein (H-antigens) components. As the O-antigen is highly variable, and structurally unique to each serotype, we investigated the binding affinities of LPS fromSalmonellaserotypes of O4 serogroup with specific anti-antigen serum via immunoblot and enzyme-linked immunosorbent assays. Since the serotypes from O4 serogroup also express the O-antigen factor 12, O12 antiserum was also used for the analysis. LPS from the different serotypes showed different binding affinities with the antisera. Therefore, based on the antigen-antibody affinity, a modified agglutination assay was carried out by using O4 and O12 antisera. Although serotypes from O4 serogroup have the common O-antigen factors 4 and 12, the analysis showed that the degree of agglutination reaction is different for each of the serotypes. We suggest thatSalmonellaserogroup O4 serotypes exhibit different binding affinities with specific antisera despite the presence of common O-antigen factors 4 and 12.