A Gold Nanocage/Cluster Hybrid Structure for Whole-Body Multispectral Optoacoustic Tomography Imaging, EGFR Inhibitor Delivery, and Photothermal Therapy.
A Gold Nanocage/Cluster Hybrid Structure for Whole-Body Multispectral Optoacoustic Tomography Imaging, EGFR Inhibitor Delivery, and Photothermal Therapy.
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DOI：
10.1002/smll.201900309
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发表时间：
2019-08
期刊：
影响因子：
13.3
通讯作者：
Chenyue Zhan;Yong Huang;Guifang Lin;Shuailing Huang;Fang Zeng;Shuizhu Wu
中科院分区：
文献类型：
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作者：
Chenyue Zhan;Yong Huang;Guifang Lin;Shuailing Huang;Fang Zeng;Shuizhu Wu
金纳米笼（AuNC）和金纳米团簇（AuClusters）是两类具有特殊光学性质的纳米结构。将它们集成到一个纳米系统中可以实现更大和更智能的性能。在这里，一个混合金纳米结构的荧光和光声断层成像，控制释放的药物，和光热治疗（PTT）的证明。对于这种纳米药物（EA-AB），将表皮生长因子受体（EGFR）抑制剂厄洛替尼（EB）加载到AuNC中，然后通过静电相互作用将其封端并通过生物相容性AuCluster@BSA（BSA =牛血清白蛋白）缀合物官能化。在细胞内化后，溶酶体蛋白酶和低pH导致EB从EA-AB释放，并且还诱导AuCluster的荧光恢复用于成像。用近红外光照射进一步促进药物释放，并提供PTT效应。基于AuNC的纳米药物具有光声活性，其生物分布和代谢过程已通过全身和3D多光谱光声断层成像成功监测。由于PTT和EGFR通路阻断的联合作用，EA-AB在体内显示出显著的肿瘤抑制功效。
Gold nanocages (AuNCs) and gold nanoclusters (AuClusters) are two classes of advantageous nanostructures with special optical properties, and many other attractive properties. Integrating them into one nanosystem may achieve greater and smarter performance. Herein, a hybrid gold nanostructure for fluorescent and optoacoustic tomography imaging, controlled release of drugs, and photothermal therapy (PTT) is demonstrated. For this nanodrug (EA-AB), an epidermal growth factor receptor (EGFR) inhibitor erlotinib (EB) is loaded into AuNCs, which are then capped and functionalized by biocompatible AuCluster@BSA (BSA = bovine serum albumin) conjugates via electrostatic interaction. Upon cell internalization, the lysosomal proteases and low pH cause the release of EB from EA-AB, and also induce fluorescence restoration of the AuCluster for imaging. Irradiation with near-infrared light further promotes the drug release and affords a PTT effect as well. The AuNC-based nanodrug is optoacoustically active, and its biodistribution and metabolic process have been successfully monitored by whole-body and 3D multispectral optoacoustic tomography imaging. Owing to the combined actions of PTT and EGFR pathway blockage, EA-AB exhibits marked tumor inhibition efficacy in vivo.