ANNUAL STABILITY IN THE LEVELS OF LYMPHOCYTE SUBPOPULATIONS IDENTIFIED BY MONOCLONAL-ANTIBODIES IN BLOOD OF HEALTHY-INDIVIDUALS
ANNUAL STABILITY IN THE LEVELS OF LYMPHOCYTE SUBPOPULATIONS IDENTIFIED BY MONOCLONAL-ANTIBODIES IN BLOOD OF HEALTHY-INDIVIDUALS
复制标题
DOI：
10.1007/bf00915163
复制
发表时间：
1985-01-01
影响因子：
9.1
通讯作者：
BLACH, CM
中科院分区：
文献类型：
--
作者：
ABO, T;MILLER, CA;BLACH, CM
在 22 个月内，每月对 2 名健康个体进行血液单核细胞中淋巴细胞、单核细胞和淋巴细胞亚群的绝对数量和百分比检查。时期。确定健康捐献者当年通过单克隆抗体、Leu4+（T）、Leu3+（辅助性 T）、Leu2+（抑制性/细胞毒性 T）和 Leu7+（自然杀伤）细胞识别的淋巴细胞亚群水平是否稳定。通过余弦方法分析结果以估计这些亚群的节律性。淋巴细胞数量变化较大，昼夜节律适中，初夏达到高峰；单核细胞的数量也有变化，但其变化没有表现出特定的节律。 T淋巴细胞亚群以及Leu3+和Leu2+细胞的绝对数呈现出协变，在初夏达到峰值，与淋巴细胞总数的昼夜节律平行。具有自然杀伤功能的颗粒淋巴细胞亚群Leu7+细胞在这一年中也表现出显着的变化。特别令人感兴趣的是 Leu3/Leu2 比率在这一年中相当稳定。 8月和1月对15名正常供者的这些淋巴细胞标志物（包括HB-2+ B细胞）进行2个时间点检查，未显示任何显着差异，尽管夏季平均值略高。这些淋巴细胞标记物的稳定性和变异性以图形方式显示，并列出了这些变异的详细信息。 。
The absolute numbers and percentages of lymphocytes, monocytes and lymphocyte subpopulations in the blood mononuclear cells were examined monthly in 2 healthy individuals over a 22-mo. period. Whether the levels of lymphocyte subpopulations identified by monoclonal antibodies, Leu4+ (T), Leu3+ (helper T), Leu2+ (suppressor/cytotoxic T) and Leu7+ (natural killer) cells, were stable during the year for healthy donors was determined. The results were analyzed by the cosiner method to estimate the rhythmicity of these subpopulations. The number of lymphocytes varied, showing a moderate circannual rhythm with a peak in early summer; the number of monocytes also varied but its variation did not show a specific rhythm. The absolute numbers of T-lymphocyte subpopulations and Leu3+ and Leu2+ cells showed a covariation with a peak in early summer in parallel with the circannual rhythm of total lymphocyte counts. A subpopulation of granular lymphocytes with natural killer function, Leu7+ cells, also showed a significant variation during the year. Of particular interest is that Leu3/Leu2 ratrios were considerably stable during the year. The 2-time point examination of these lymphocyte markers including HB-2+ B cells in Aug and Jan in 15 normal donors did not show any significant differences, although the mean values were slightly higher in summer. The stability and variability of these lymphocyte markers are displayed graphically and the details of these variations are listed. .